High throughput fabrication of cell spheroids by templating water-in-water Pickering emulsions

被引：29
作者：
Das, Anupam A. K. [1 ]
Filby, Benjamin W. [1 ]
Geddes, Daniel A. [1 ]
Legrande, Deborah [1 ]
Paunov, Vesselin N. [1 ]
机构：
[1] Univ Hull, Sch Math & Phys Sci Chem, Cottingham Rd, Kingston Upon Hull HU6 7RX, N Humberside, England
来源：
MATERIALS HORIZONS | 2017年 / 4卷 / 06期
关键词：
FLUORESCEIN DIACETATE; STABILIZATION; BACTERIA; CULTURE; 3-D;
D O I：
10.1039/c7mh00677b
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report a simple technique for the high throughput generation of tissue spheroids based on the encapsulation of dispersed adherent cells in particle-stabilised water-in-water emulsions. We demonstrate cell encapsulation and spheroid generation using fibroblast cells which can find applications in 3D culturing, tissue engineering, inks for bioprinting and cell therapy.
引用
收藏
页码：1196 / 1200
页数：5
相关论文
共 28 条
  • [1] Label-free cell aggregate formation based on the magneto-Archimedes effect
    Akiyama, Yoshitake
    Morishima, Keisuke
    [J]. APPLIED PHYSICS LETTERS, 2011, 98 (16)
  • [2] Directed assembly of yeast cells into living yeastosomes by microbubble templating
    Brandy, Marie-Laure
    Cayre, Olivier J.
    Fakhrullin, Rawil F.
    Velev, Orlin D.
    Paunov, Vesselin N.
    [J]. SOFT MATTER, 2010, 6 (15) : 3494 - 3498
  • [3] A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester
    Breeuwer, P
    Drocourt, JL
    Rombouts, FM
    Abee, T
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (01) : 178 - 183
  • [4] CHARACTERIZATION OF UPTAKE AND HYDROLYSIS OF FLUORESCEIN DIACETATE AND CARBOXYFLUORESCEIN DIACETATE BY INTRACELLULAR ESTERASES IN SACCHAROMYCES-CEREVISIAE, WHICH RESULT IN ACCUMULATION OF FLUORESCENT PRODUCT
    BREEUWER, P
    DROCOURT, JL
    BUNSCHOTEN, N
    ZWIETERING, MH
    ROMBOUTS, FM
    ABEE, T
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (04) : 1614 - 1619
  • [5] APPLICABILITY OF THE FLUORESCEIN DIACETATE METHOD OF DETECTING ACTIVE BACTERIA IN FRESH-WATER
    CHRZANOWSKI, TH
    CROTTY, RD
    HUBBARD, JG
    WELCH, RP
    [J]. MICROBIAL ECOLOGY, 1984, 10 (02) : 179 - 185
  • [6] Das AAK, 2014, RSC SMART MATER, V9, P162
  • [7] Live celloidosome structures based on the assembly of individual cells by colloid interactions
    Fakhrullin, Rawil F.
    Brandy, Marie-Laure
    Cayre, Olivier J.
    Velev, Orlin D.
    Paunov, Vesselin N.
    [J]. PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2010, 12 (38) : 11912 - 11922
  • [8] Experimental anti-tumor therapy in 3-D: Spheroids - old hat or new challenge?
    Friedrich, Juergen
    Ebner, Reinhard
    Kunz-Schughart, Leoni A.
    [J]. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2007, 83 (11-12) : 849 - 871
  • [9] Stabilisation of water-in-water emulsions by montmorillonite platelets
    Ganley, William J.
    Ryan, Paul T.
    van Duijneveldt, Jeroen S.
    [J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2017, 505 : 139 - 147
  • [10] Natural polymers for the microencapsulation of cells
    Gasperini, Luca
    Mano, Joao F.
    Reis, Rui L.
    [J]. JOURNAL OF THE ROYAL SOCIETY INTERFACE, 2014, 11 (100)
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