A self-powered glucose biosensor device based on microfluidics using human blood

A self-powered glucose biosensor (SPGB) was developed based on biofuel cells (BFCs)and a microfluidic system as a suitable technology for high potential application in clinical diagnosis. In this work, a bioanode and biocathode were assembled by glucose oxidase-based and laccase-based electrodes, respectively, which were incorporated into a microfluidic system (m-SPGB), while the anolyte and catholyte were phosphate buffered at different pH values of 7.4 and 5.6, respectively. The m-SPGB showed a linear dynamic interval of 0-10 mM (R2 = 0.9907), a low limit of detection of 0.48 mM and a response to interfering species. Subsequently, human blood was used in the anolyte to quantify glucose molecules in real samples, obtaining a low relative error percentage when compared with a commercial glucometer. The coupling of the m-SPGB with a wireless electronic device allowed the detection and processing of the signal in the quantification of glucose directly without using a potentiostat/galvanostat. The promising results of the m-SPGB connected to a Wi-Fi electronic device are well suited for clinical application.