Determining Carbon Kinetic Isotope Effects Using Headspace Analysis of Evolved CO2

被引：1

作者：

Mundle, Scott O. C. ^{[1
]}

Lollar, Barbara Sherwood ^{[2
]}

Kluger, Ronald ^{[2
]}

机构：

[1] Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON, Canada

[2] Univ Toronto, Toronto, ON, Canada

来源：

MEASUREMENT AND ANALYSIS OF KINETIC ISOTOPE EFFECTS | 2017年 / 596卷

关键词：

ENZYME-CATALYZED DECARBOXYLATIONS; RATIO MASS-SPECTROMETRY; BUTYL RUBBER STOPPERS; GLUTAMATE-DECARBOXYLASE; ANAEROBIC DEGRADATION; CHLORINATED ETHENES; GAS-CHROMATOGRAPHY; FRACTIONATION; ACID; BIODEGRADATION;

D O I：

10.1016/bs.mie.2017.07.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isotope ratio mass spectrometry (IRMS) provides accurate measurements of relative abundance of isotopes of heavy atoms for reactions that are subject to kinetic isotope effects (KIEs). The recent development of compound-specific isotope analysis (CSIA) allows the use of multiple time points that provide data for a rate plot as well as isotope ratios. Utilizing CSIA in enzymology presents opportunities for obtaining heavy atom KIEs in diverse areas.

引用

页码：501 / 522

页数：22

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