Polyethylene Glycol 35 (PEG35) Protects against Inflammation in Experimental Acute Necrotizing Pancreatitis and Associated Lung Injury

被引:21
|
作者
Ferrero-Andres, Ana [1 ]
Panisello-Rosello, Arnau [1 ]
Serafin, Anna [2 ]
Rosello-Catafau, Joan [3 ]
Folch-Puy, Emma [3 ]
机构
[1] CSIC, IIBB, Expt Pathol Dept, Barcelona 08036, Catalonia, Spain
[2] Parc Cient Barcelona, PCB Anim Facil, Barcelona 08028, Catalonia, Spain
[3] CSIC, Ctr Invest Biomed Red Enfermedades Hepat & Digest, Inst Invest Biomed August Pi & Sunyer IDIBAPS, Expt Pathol Dept,IIBB, Barcelona 08036, Catalonia, Spain
关键词
pancreatitis; inflammation; pulmonary injury; polyethylene glycols; cytokines; CELL-DEATH; BIOPHARMACEUTICALS;
D O I
10.3390/ijms21030917
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acute pancreatitis is an inflammatory disorder of the pancreas. Its presentation ranges from self-limiting disease to acute necrotizing pancreatitis (ANP) with multiorgan failure and a high mortality. Polyethylene glycols (PEGs) are non-immunogenic, non-toxic, and water-soluble chemicals composed of repeating units of ethylene glycol. The present article explores the effect of PEG35 administration on reducing the severity of ANP and associated lung injury. ANP was induced by injection of 5% sodium taurocholate into the biliopancreatic duct. PEG35 was administered intravenously either prophylactically or therapeutically. Three hours after ANP induction, pancreas and lung tissue samples and blood were collected and ANP severity was assessed. To evaluate the inflammatory response, gene expression of pro-inflammatory cytokines and chemokine and the changes in the presence of myeloperoxidase and adhesion molecule levels were determined in both the pancreas and the lung. To evaluate cell death, lactate dehydrogenase (LDH) activity and apoptotic cleaved caspase-3 localization were determined in plasma and in both the pancreatic and lung tissue respectively. ANP-associated local and systemic inflammatory processes were reduced when PEG35 was administered prophylactically. PEG35 pre-treatment also protected against acute pancreatitis-associated cell death. Notably, the therapeutic administration of PEG35 significantly decreased associated lung injury, even when the pancreatic lesion was equivalent to that in the untreated ANP-induced group. Our results support a protective role of PEG35 against the ANP-associated inflammatory process and identify PEG35 as a promising tool for the treatment of the potentially lethal complications of the disease.
引用
收藏
页数:16
相关论文
共 50 条
  • [1] Polyethylene Glycol 35 (PEG35) Modulates Exosomal Uptake and Function
    Ferrero-Andres, Ana
    Closa, Daniel
    Rosello-Catafau, Joan
    Folch-Puy, Emma
    POLYMERS, 2020, 12 (12) : 1 - 13
  • [2] PEG35 as a Preconditioning Agent against Hypoxia/Reoxygenation Injury
    Teixeira da Silva, Rui
    Machado, Ivo F.
    Teodoro, Joao S.
    Panisello-Rosello, Arnau
    Rosello-Catafau, Joan
    Rolo, Anabela P.
    Palmeira, Carlos M.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (03)
  • [3] Resolvin D1 protects against inflammation in experimental acute pancreatitis and associated lung injury
    Liu, Yong
    Zhou, Dan
    Long, Fei-Wu
    Chen, Ke-Ling
    Yang, Hong-Wei
    Lv, Zhao-Yin
    Zhou, Bin
    Peng, Zhi-Hai
    Sun, Xiao-Feng
    Li, Yuan
    Zhou, Zong-Guang
    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2016, 310 (05): : G303 - G309
  • [4] Lung inflammation associated with acute necrotizing pancreatitis in dogs
    Vrolyk, Vanessa
    Wobeser, Bruce
    Al-Dissi, Ahmad
    Carr, Anthony
    Singh, Baljit
    FASEB JOURNAL, 2014, 28 (01):
  • [5] Lung Inflammation Associated With Clinical Acute Necrotizing Pancreatitis in Dogs
    Vrolyk, V.
    Wobeser, B. K.
    Al-Dissi, A. N.
    Carr, A.
    Singh, B.
    VETERINARY PATHOLOGY, 2017, 54 (01) : 129 - 140
  • [6] Effects of Berberine on Acute Necrotizing Pancreatitis and Associated Lung Injury
    Choi, Sun-Bok
    Bae, Gi-Sang
    Jo, Il-Joo
    Song, Ho-Joon
    Park, Sung-Joo
    PANCREAS, 2017, 46 (08) : 1046 - 1055
  • [7] Glycyrrhizic Acid Protects Experimental Sepsis Rats against Acute Lung Injury and Inflammation
    Shen, Jiali
    Hua, Zhongjie
    Chai, Yueyang
    EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2022, 2022
  • [8] Eosinophil Protects Against the Onset of Inflammation in Acute Lung Injury
    Zhu, C.
    Zhou, L.
    Zhao, Y.
    Li, F.
    Wu, Y.
    Wu, Y.
    Li, Z.
    Li, M.
    Weng, Q.
    Hu, Y.
    Zhang, C.
    Ying, S.
    Chen, Z.
    Shen, H.
    Li, W.
    AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2018, 197
  • [9] Beneficial Effects of Berberine on Acute Necrotizing Pancreatitis and Associated Lung Injury
    Bae, G. -S.
    Park, S. -J.
    Kim, D. -G.
    Kim, M. J.
    Choi, S.
    Jeong, J. H.
    PANCREAS, 2016, 45 (10) : 1496 - 1496
  • [10] Melatonin protects against acute obstructive pancreatitis and associated lung injury in rats: Role of neutrophils
    Barlas, A
    Cevik, H
    Tekin, S
    Arbak, S
    Sener, G
    Yegen, C
    Yegen, BC
    GASTROENTEROLOGY, 2004, 126 (04) : A384 - A384