Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1

被引：29

作者：

Quejada, Jose R. ^{[1
,2
,3
]}

Park, Seon-Hye E. ^{[1
,2
,7
]}

Awari, Daniel W. ^{[1
,2
,8
]}

Shi, Fan ^{[1
,2
,4
]}

Yamamoto, Hannah E. ^{[1
,2
,5
]}

Kawano, Fuun ^{[1
,2
]}

Jung, Juergen C. ^{[6
,9
]}

Yazawa, Masayuki ^{[1
,2
,3
]}

机构：

[1] Columbia Univ, Columbia Stem Cell Initiat, New York, NY 10032 USA

[2] Columbia Univ, Dept Rehabil & Regenerat Med, New York, NY 10032 USA

[3] Columbia Univ, Dept Pharmacol, New York, NY 10032 USA

[4] Tianjin Univ, Coll Precis Instrument & Optoelect Engn, Tianjin 300072, Peoples R China

[5] Barnard Coll, New York, NY 10027 USA

[6] Stanford Univ, Dept Biol, Stanford, CA 94305 USA

[7] Univ Texas Southwestern Med Ctr, Dallas, TX 75390 USA

[8] Rowan Univ, Sch Osteopath Med, Stratford, NJ 08084 USA

[9] Edison Pharmaceut, Mountain View, CA 94043 USA

来源：

NUCLEIC ACIDS RESEARCH | 2017年 / 45卷 / 20期

基金：

日本科学技术振兴机构; 美国国家卫生研究院;

关键词：

SPATIOTEMPORAL CONTROL; PROTEIN INTERACTIONS; GENE-EXPRESSION; OPTICAL CONTROL; LIVING CELLS; BLUE-LIGHT; ACTIVATION; SYSTEM; INDICATORS; INDUCTION;

D O I：

10.1093/nar/gkx804

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (Gl) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/Gland CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.

引用

页数：12

共 1 条

[1] Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms
Liu, Hongtao
Wang, Qin
Liu, Yawen
Zhao, Xiaoying
Imaizumi, Takato
Somers, David E.
Tobin, Elaine M.
Lin, Chentao
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (43) : 17582 - 17587

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