Heteronuclear 2D-correlations in a uniformly [13C, 15N] labeled membrane-protein complex at ultra-high magnetic fields

被引：81

作者：

Egorova-Zachernyuk, TA

Hollander, J

Fraser, N

Gast, P

Hoff, AJ

Cogdell, R

de Groot, HJM ^{[1
]}

Baldus, M

机构：

[1] Leiden Univ, Gorlaeus Labs, NL-2333 CC Leiden, Netherlands

[2] Leiden Univ, Huygens Lab, NL-2333 CC Leiden, Netherlands

[3] Univ Glasgow, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland

来源：

JOURNAL OF BIOMOLECULAR NMR | 2001年 / 19卷 / 03期

关键词：

correlation spectroscopy; LH2 light-harvesting complex; MAS; membrane-proteins; photosynthetic bacteria; polarization transfer; spin-diffusion; solid-state NMR;

D O I：

10.1023/A:1011235417465

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

One- and two-dimensional solid-state NMR experiments on a uniformly labeled intrinsic membrane-protein complex at ultra-high magnetic fields are presented. Two-dimensional backbone and side-chain correlations for a [U-C-13,N-15] labeled version of the LH2 light-harvesting complex indicate significant resolution at low temperatures and under Magic Angle Spinning. Tentative assignments of some of the observed correlations are presented and attributed to the alpha -helical segments of the protein, mostly found in the membrane interior.

引用

页码：243 / 253

页数：11

共 58 条

[21]

HAWTHORNTHWAITE AM, 1991, CHLOROPHYLLS, P493

[22] ADIABATIC PASSAGE HARTMANN-HAHN CROSS-POLARIZATION IN NMR UNDER MAGIC-ANGLE SAMPLE-SPINNING [J].

HEDIGER, S ;

MEIER, BH ;

ERNST, RR .

CHEMICAL PHYSICS LETTERS, 1995, 240 (5-6) :449-456

[23] Fivefold symmetric homonuclear dipolar recoupling in rotating solids: Application to double quantum spectroscopy [J].

Hohwy, M ;

Rienstra, CM ;

Jaroniec, CP ;

Griffin, RG .

JOURNAL OF CHEMICAL PHYSICS, 1999, 110 (16) :7983-7992

[24] Resonance assignment of 13C/15N labeled solid proteins by two- and three-dimensional magic-angle-spinning NMR [J].

Hong, M .

JOURNAL OF BIOMOLECULAR NMR, 1999, 15 (01) :1-14

[25] Selective and extensive 13C labeling of a membrane protein for solid-state NMR investigations [J].

Hong, M ;

Jakes, K .

JOURNAL OF BIOMOLECULAR NMR, 1999, 14 (01) :71-74

[26] 3-DIMENSIONAL TRIPLE-RESONANCE NMR-SPECTROSCOPY OF ISOTOPICALLY ENRICHED PROTEINS [J].

KAY, LE ;

IKURA, M ;

TSCHUDIN, R ;

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1990, 89 (03) :496-514

[27] High-resolution polypeptide structure in a lamellar phase lipid environment from solid state NMR derived orientational constraints [J].

Ketchem, RR ;

Roux, B ;

Cross, TA .

STRUCTURE, 1997, 5 (12) :1655-1669

[28] HIGH-RESOLUTION CONFORMATION OF GRAMICIDIN-A IN A LIPID BILAYER BY SOLID-STATE NMR [J].

KETCHEM, RR ;

HU, W ;

CROSS, TA .

SCIENCE, 1993, 261 (5127) :1457-1460

[29] Closed form of liganded glutamine-binding protein by rotational-echo double-resonance NMR [J].

Klug, CA ;

Tasaki, K ;

Tjandra, N ;

Ho, C ;

Schaefer, J .

BIOCHEMISTRY, 1997, 36 (31) :9405-9408

[30] SPECTRAL SPIN DIFFUSION IN POLYCRYSTALLINE SOLIDS UNDER MAGIC-ANGLE SPINNING [J].

KUBO, A ;

MCDOWELL, CA .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1988, 84 :3713-3730

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