Off-target effects of cytidine base editor and adenine base editor: What can we do?

被引：2
|
作者：
Liang, Puping [1 ,2 ,3 ,4 ]
Wen, Jinkun [1 ,2 ,3 ,4 ,5 ]
Huang, Junjiu [1 ,2 ,3 ,4 ,6 ]
机构：
[1] Sun Yat Sen Univ, Sch Life Sci, Key Lab Reprod Med Guangdong Prov, Guangzhou 510275, Peoples R China
[2] Sun Yat Sen Univ, Affiliated Hosp 1, Guangzhou 510275, Peoples R China
[3] Sun Yat Sen Univ, Sch Life Sci, SYSU BCM Joint Res Ctr, MOE Key Lab Gene Funct & Regulat, Guangzhou 510275, Peoples R China
[4] Sun Yat Sen Univ, Sch Life Sci, SYSU BCM Joint Res Ctr, Guangzhou Key Lab Hlth Aging Res, Guangzhou 510275, Peoples R China
[5] Sun Yat Sen Univ, Jiangmen Cent Hosp, Affiliated Jiangmen Hosp, Guangzhou 510150, Peoples R China
[6] Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Reprod Med Guangdong Prov, Guangzhou 510150, Peoples R China
来源：
JOURNAL OF GENETICS AND GENOMICS | 2019年 / 46卷 / 11期
基金：
国家重点研发计划;
关键词：
GENOMIC DNA; RNA; DEAMINASE;
D O I：
10.1016/j.jgg.2019.09.004
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
[No abstract available]
引用
收藏
页码：509 / 512
页数：4
相关论文
共 50 条
  • [41] Functional restoration of a CFTR splicing mutation through RNA delivery of CRISPR adenine base editor
    Amistadi, Simone
    Maule, Giulia
    Ciciani, Matteo
    Ensinck, Marjolein M.
    De Keersmaecker, Liesbeth
    Ramalho, Anabela S.
    Guidone, Daniela
    Buccirossi, Martina
    Galietta, Luis J. V.
    Carlon, Marianne S.
    Cereseto, Anna
    MOLECULAR THERAPY, 2023, 31 (06) : 1647 - 1660
  • [42] CRISPR DNA base editors with reduced RNA off-target and self-editing activities
    Grunewald, Julian
    Zhou, Ronghao
    Iyer, Sowmya
    Lareau, Caleb A.
    Garcia, Sara P.
    Aryee, Martin J.
    Joung, J. Keith
    NATURE BIOTECHNOLOGY, 2019, 37 (09) : 1041 - +
  • [43] Global quantification exposes abundant low-level off-target activity by base editors
    Buchumenski, Ilana
    Roth, Shalom Hillel
    Kopel, Eli
    Katsman, Efrat
    Feiglin, Ariel
    Levanon, Erez Y.
    Eisenberg, Eli
    GENOME RESEARCH, 2021, 31 (12) : 2354 - 2361
  • [44] A cytosine base editor toolkit with varying activity windows and target scopes for versatile gene manipulation in plants
    Xiong, Xiangyu
    Li, Zhenxiang
    Liang, Jieping
    Liu, Kehui
    Li, Chenlong
    Li, Jian-Feng
    NUCLEIC ACIDS RESEARCH, 2022, 50 (06) : 3565 - 3580
  • [45] Off-Targeting of Base Editors: BE3 but not ABE induces substantial off-target single nucleotide variants
    Xin, Huhu
    Wan, Tao
    Ping, Yuan
    SIGNAL TRANSDUCTION AND TARGETED THERAPY, 2019, 4 (1)
  • [46] High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants
    Fan, Tingting
    Cheng, Yanhao
    Wu, Yuechao
    Liu, Shishi
    Tang, Xu
    He, Yao
    Liao, Shanyue
    Zheng, Xuelian
    Zhang, Tao
    Qi, Yiping
    Zhang, Yong
    NATURE COMMUNICATIONS, 2024, 15 (01)
  • [47] Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli
    Shelake, Rahul Mahadev
    Pramanik, Dibyajyoti
    Kim, Jae-Yean
    MBIO, 2023, 14 (01):
  • [48] Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors
    Doman, Jordan L.
    Raguram, Aditya
    Newby, Gregory A.
    Liu, David R.
    NATURE BIOTECHNOLOGY, 2020, 38 (05) : 620 - +
  • [49] Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations
    Seys, Francois M.
    Humphreys, Christopher M.
    Tomi-Andrino, Claudio
    Li, Qi
    Millat, Thomas
    Yang, Sheng
    Minton, Nigel P.
    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2023, 11
  • [50] Cost-effective generation of A-to-G mutant mice by zygote electroporation of adenine base editor ribonucleoproteins
    Sun, Hongwei
    Zhi, Shengyao
    Wu, Guifang
    Wu, Guanglan
    Cao, Tianqi
    Hao, Hu
    Zhou Songyang
    Liang, Puping
    Huang, Junjiu
    JOURNAL OF GENETICS AND GENOMICS, 2020, 47 (06) : 337 - 340
12345