Effect of an antifungal peptide from oyster enzymatic hydrolysates for control of gray mold (Botrytis cinerea) on harvested strawberries

被引:52
作者
Liu, Zunying [1 ]
Zeng, Mingyong [1 ]
Dong, Shiyuan [1 ]
Xu, Jie [1 ]
Song, Hongxia [1 ]
Zhao, Yuanhui [1 ]
机构
[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
关键词
antifungal peptides; gray mold; Botrytis cinerea; strawberry; oyster;
D O I
10.1016/j.postharvbio.2007.03.013
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
A novel peptide CgPep33, with high inhibitory activity against Botrytis cinerea growth has been isolated from enzymatic hydrolysates from the Pacific oyster (Crassostrea gigas), and purified by DEAE Sephadex A-25 ion exchange, Sephadex G-25 gel filtration and HPLC. CgPep33 inhibited the in vitro growth of B. cinerea by 50% at 20-40 mu g mL(-1) and by 100% at 120 mu g mL(-1). The disease incidence and lesion diameter of CgPep33-treated fruit were 76% and 6.3 mm, respectively, lower than that of the fruit without CgPep33 treatment on the 3rd day after inoculation. The results suggest that this peptide should provide an alternative to fungicides for controlling postharvest disease. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:95 / 98
页数:4
相关论文
共 10 条
[1]   Inhibition of fungal and bacterial plant pathogens by synthetic peptides: in vitro growth inhibition, interaction between peptides and inhibition of disease progression [J].
Ali, GS ;
Reddy, ASN .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (08) :847-+
[2]   Antibacterial activities of oyster (Crassostrea virginica) and mussel (Mytilus edulis and Geukensia demissa) plasma [J].
Anderson, RS ;
Beaven, AE .
AQUATIC LIVING RESOURCES, 2001, 14 (06) :343-349
[3]   Cecropin A-derived peptides are potent inhibitors of fungal plant pathogens [J].
Cavallarin, L ;
Andreu, D ;
Segundo, BS .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (03) :218-227
[4]   Expression of an antifungal peptide in Saccharomyces:: A new approach for biological control of the postharvest disease caused by Colletotrichum coccodes [J].
Jones, RW ;
Prusky, D .
PHYTOPATHOLOGY, 2002, 92 (01) :33-37
[5]   Comparison of the activity of antifungal hexapeptides and the fungicides thiabendazole and imazalil against postharvest fungal pathogens [J].
López-García, B ;
Veyrat, A ;
Pérez-Payá, E ;
González-Candelas, L ;
Marcos, JF .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 89 (2-3) :163-170
[6]   Identification and characterization of a hexapeptide with activity against phytopathogenic fungi that cause postharvest decay in fruits [J].
López-García, B ;
González-Candelas, L ;
Pérez-Payá, E ;
Marcos, JF .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (08) :837-846
[7]   SYNTHETIC ANTIMICROBIAL PEPTIDE DESIGN [J].
POWELL, WA ;
CATRANIS, CM ;
MAYNARD, CA .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1995, 8 (05) :792-794
[8]   From "carpet" mechanism to de-novo designed diastereomeric cell-selective antimicrobial peptides [J].
Shai, Y ;
Oren, Z .
PEPTIDES, 2001, 22 (10) :1629-1641
[9]   A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein [J].
Tailor, RH ;
Acland, DP ;
Attenborough, S ;
Cammue, BPA ;
Evans, IJ ;
Osborn, RW ;
Ray, JA ;
Rees, SB ;
Broekaert, WF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (39) :24480-24487
[10]   SMALL CYSTEINE-RICH ANTIFUNGAL PROTEINS FROM RADISH - THEIR ROLE IN HOST-DEFENSE [J].
TERRAS, FRG ;
EGGERMONT, K ;
KOVALEVA, V ;
RAIKHEL, NV ;
OSBORN, RW ;
KESTER, A ;
REES, SB ;
TORREKENS, S ;
VANLEUVEN, F ;
VANDERLEYDEN, J ;
CAMMUE, BPA ;
BROEKAERT, WF .
PLANT CELL, 1995, 7 (05) :573-588