Bench-scale evaluation of in situ bioremediation strategies for soil at a former manufactured gas plant site

被引:17
作者
Li, J
Pignatello, JJ [1 ]
Smets, BF
Grasso, D
Monserrate, E
机构
[1] Connecticut Agr Expt Stn, Dept Soil & Water, New Haven, CT 06504 USA
[2] Univ Connecticut, Dept Civil & Environm Engn, Storrs, CT 06269 USA
[3] Univ Connecticut, Dept Mol & Cell Biol, Storrs, CT 06269 USA
[4] Smith Coll, Picker Engn Program, Northampton, MA 01063 USA
[5] Smith Coll, Dept Biol Sci, Northampton, MA 01063 USA
关键词
bioremediation; bioavailability; coal tar contamination; nutrient limitation; polycyclic aromatic hydrocarbons;
D O I
10.1897/04-247R.1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
We examined the biodegradation and desorption of a set of 15 polycyclic aromatic hydrocarbon (PAH) compounds in coal tar-contaminated soil at a former manufactured gas plant site to evaluate the feasibility of in situ bioremediation. Experiments were conducted in well-mixed aerobic soil suspensions containing various additives over a 93- to 106-d period. In general, both biotransformation and desorption decreased with PAH ring size, becoming negligible for the six-ring PAH compounds. Biodegradation by indigenous microorganisms was strongly accelerated by addition of inorganic nutrients (N, P, K, and trace metals). The rates of biotransformation of PAH compounds by indigenous microorganisms in nutrient-amended flasks outpaced their maximum (i.e., chelate-enhanced) rates of desorption to an infinite sink (Tenax(R)) in sterilized systems run in parallel, suggesting that indigenous organisms facilitated desorption. Biodegradation by indigenous organisms in nutrient-amended flasks appeared to be unaffected by the addition of a site-derived bacterial enrichment culture, resulting in approximately 100-fold higher aromatic dioxygenase levels, and by the addition of 0.01 M chelating agent (citrate or pyrophosphate), although such chelating agents greatly enhanced desorption in microbially inactivated flasks. The strong ability of nutrients to enhance degradation of the bioavailable PAHs indicates that their persistence for many decades at this site likely results from nutrient-limited natural biodegradation, and it also suggests that an effective strategy for their bioremediation could consist simply of adding inorganic nutrients.
引用
收藏
页码:741 / 749
页数:9
相关论文
共 33 条
[21]  
0631:EOBIAN&gt
[22]  
2.3.CO
[23]  
2, 10.1002/etc.5620160405]
[24]   Degradation of polynuclear aromatic hydrocarbons under bench-scale compost conditions [J].
Potter, CL ;
Glaser, JA ;
Chang, LW ;
Meier, JR ;
Dosani, MA ;
Herrmann, RF .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1999, 33 (10) :1717-1725
[25]   Distributed sequestration and release of PAHs in weathered sediment: The role of sediment structure and organic carbon properties [J].
Rockne, KJ ;
Shor, LM ;
Young, LY ;
Taghon, GL ;
Kosson, DS .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (12) :2636-2644
[26]   Polycyclic aromatic hydrocarbons (PAHs) slurry phase bioremediation of a manufacturing gas plant (MGP) site aged soil [J].
Saponaro, S ;
Bonomo, L ;
Petruzzelli, G ;
Romele, L ;
Barbafieri, M .
WATER AIR AND SOIL POLLUTION, 2002, 135 (1-4) :219-236
[27]   Land treatment of PAH contaminated soil: Performance measured by chemical and toxicity assays [J].
Sayles, GD ;
Acheson, CM ;
Kupferle, MJ ;
Shan, Y ;
Zhou, Q ;
Meier, JR ;
Chang, L ;
Brenner, RC .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1999, 33 (23) :4310-4317
[28]   Enhancement of polynuclear aromatic hydrocarbon desorption by complexing agents in weathered soil [J].
Subramaniam, K ;
Stepp, C ;
Pignatello, JJ ;
Smets, B ;
Grasso, D .
ENVIRONMENTAL ENGINEERING SCIENCE, 2004, 21 (04) :515-523
[29]   Particle-scale understanding of the bioavailability of PAHs in sediment [J].
Talley, JW ;
Ghosh, U ;
Tucker, SG ;
Furey, JS ;
Luthy, RG .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2002, 36 (03) :477-483
[30]   Biodegradation of aromatic compounds under mixed oxygen/denitrifying conditions: A review [J].
Wilson, LP ;
Bouwer, EJ .
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1997, 18 (2-3) :116-130