Design, Synthesis and Characterization of a New Anionic Cell-Penetrating Peptide: SAP(E)

被引：68

作者：

Martin, Irene ^{[1
]}

Teixido, Meritxell ^{[1
]}

Giralt, Ernest ^{[1
,2
]}

机构：

[1] Inst Res Biomed IRB Barcelona, Barcelona 08028, Spain

[2] Univ Barcelona, Dept Organ Chem, E-08028 Barcelona, Spain

来源：

CHEMBIOCHEM | 2011年 / 12卷 / 06期

关键词：

cell-penetrating peptides; drug delivery; peptides; protein transduction domain; sweet arrow peptide; MEDIATED INTRACELLULAR DELIVERY; ARGININE-RICH PEPTIDES; MOLECULAR-MECHANISMS; STRUCTURAL DOMAINS; TAT PEPTIDE; PROTEINS; INTERNALIZATION; TRANSLOCATION; RETENTION; HELIX;

D O I：

10.1002/cbic.201000679

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-penetrating peptides (CPPs) are powerful tools to transport cell-impermeable cargoes into the cytoplasm without damaging the cell membrane. The vast majority of these peptides described to date share several features, among others, they are positively charged at physiological pH. In several cases a clear correlation between an increasing number of positive charges and internalization properties has been reported. Here, we describe what, to the best of our knowledge, is the first anionic CPP. This new compound SAP(E) internalizes into a range of cell lines with good efficiency and it shows low toxicity. We also report on the internalization mechanism. The discovery of this new class of CPP opens the way to the intracellular delivery of new molecular cargoes.

引用

页码：896 / 903

页数：8

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