Etiolation of 'Royal Gala' apple (Malus x domestica Borkh.) shoots promotes high-frequency shoot organogenesis and enhanced β-glucuronidase expression from stem internodes

被引：21

作者：

Liu, Q ^{[1
]}

Salih, S ^{[1
]}

Hammerschlag, F ^{[1
]}

机构：

[1] ARS, USDA, Fruit Lab, Beltsville, MD 20705 USA

来源：

PLANT CELL REPORTS | 1998年 / 18卷 / 1-2期

关键词：

Agrobacterium tumefaciens; gusA expression; transformation;

D O I：

10.1007/s002990050527

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Internodal explants from etiolated 'Royal Gala' apple shoots were compared with those from non-etiolated shoots for frequency of shoot organogenesis and for efficiency of beta-glucuronidase (GUS) expression after cocultivation of explants with Agrobacterium tumefaciens strain EHA105 (p35SGUSint). First (youngest) internodal ex plants from etiolated shoots produced 2-, 8- and 73-fold numbers of shoots compared to second, third, and fourth internodal explants, respectively. Moreover, these explants produced sevenfold the number of shoots as similar explants from non-etiolated shoots. All first internodes from etiolated shoots exhibited GUS-expressing zones and yielded fourfold as many GUS-expressing zones as commonly used leaf explants from non-etiolated shoots, which exhibited GUS-expressing zones in only 63% of the explants. An average of 9.8 GUS expressing calli per explant were observed on first internodes from etiolated shoots 2 weeks after cocultivation with A. tumefaciens.

引用

页码：32 / 36

页数：5

共 27 条

[1]

AKAMA K, 1992, PLANT CELL REP, V12, P7, DOI 10.1007/BF00232413

[2]

BELAIZI M, 1991, PLANT CELL REP, V9, P471, DOI 10.1007/BF00232098

[3]

Brown A. G., 1975, Advances in fruit breeding. Temperate fruits., P3

[4]

CHU CC, 1975, SCI SINICA, V18, P659