Structure of astacin with a transition-state analogue inhibitor

被引：140

作者：

Grams, F

Dive, V

Yiotakis, A

Yiallouros, I

Vassiliou, S

Zwilling, R

Bode, W

Stocker, W

机构：

[1] UNIV HEIDELBERG,INST ZOOL,D-69120 HEIDELBERG,GERMANY

[2] MAX PLANCK INST BIOCHEM,ABT STRUKTURFORSCH,D-82152 PLANEGG,GERMANY

[3] CENS,CEA,DEPT INGN& ETUD PROT,F-91191 GIF SUR YVETTE,FRANCE

[4] UNIV ATHENS,DEPT ORGAN CHEM,ZOGRATU 15771,ATHENS,GREECE

来源：

NATURE STRUCTURAL BIOLOGY | 1996年 / 3卷 / 08期

关键词：

D O I：

10.1038/nsb0896-671

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the zinc peptidase astacin in complex with a phosphinic peptide suggests that a special role is played by the side chain of a zinc-bound tyrosine, which is shifted to form bond to the phosphinyl group-a mimic of the carboxyanion of the transition state.

引用

收藏

页码：671 / 675

页数：5

相关论文

共 29 条

[1]

Auld D.S., 1987, HYDROLYTIC ENZYMES, P201

[2] EVALUATION OF INTRINSIC BINDING-ENERGY FROM A HYDROGEN-BONDING GROUP IN AN ENZYME-INHIBITOR [J].

BARTLETT, PA ;

MARLOWE, CK .

SCIENCE, 1987, 235 (4788) :569-571

[3] 3-DIMENSIONAL STRUCTURE OF THE ALKALINE PROTEASE OF PSEUDOMONAS-AERUGINOSA - A 2-DOMAIN PROTEIN WITH A CALCIUM-BINDING PARALLEL-BETA ROLL MOTIF [J].

BAUMANN, U ;

WU, S ;

FLAHERTY, KM ;

MCKAY, DB .

EMBO JOURNAL, 1993, 12 (09) :3357-3364

[4] CRYSTAL-STRUCTURE OF THE 50 KDA METALLOPROTEASE FROM SERRATIA-MARCESCENS [J].

BAUMANN, U .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 242 (03) :244-251

[5] CRYSTAL-STRUCTURE OF A COMPLEX BETWEEN SERRATIA-MARCESCENS METALLOPROTEASE AND AN INHIBITOR FROM ERWINIA-CHRYSANTHEMI [J].

BAUMANN, U ;

BAUER, M ;

LETOFFE, S ;

DELEPELAIRE, P ;

WANDERSMAN, C .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (03) :653-661

[6] THE ROLE OF HISTIDINE-231 IN THERMOLYSIN-LIKE ENZYMES - A SITE-DIRECTED MUTAGENESIS STUDY [J].

BEAUMONT, A ;

ODONOHUE, MJ ;

PAREDES, N ;

ROUSSELET, N ;

ASSICOT, M ;

BOHUON, C ;

FOURNIEZALUSKI, MC ;

ROQUES, BP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (28) :16803-16808

[7] STRUCTURE OF ASTACIN AND IMPLICATIONS FOR ACTIVATION OF ASTACINS AND ZINC-LIGATION OF COLLAGENASES [J].

BODE, W ;

GOMISRUTH, FX ;

HUBER, R ;

ZWILLING, R ;

STOCKER, W .

NATURE, 1992, 358 (6382) :164-167

[8] THE ASTACIN FAMILY OF METALLOENDOPEPTIDASES [J].

BOND, JS ;

BEYNON, RJ .

PROTEIN SCIENCE, 1995, 4 (07) :1247-1261

[9] CRYSTALLOGRAPHIC REFINEMENT BY SIMULATED ANNEALING - APPLICATION TO CRAMBIN [J].

BRUNGER, AT ;

KARPLUS, M ;

PETSKO, GA .

ACTA CRYSTALLOGRAPHICA SECTION A, 1989, 45 :50-61

[10] CARBOXYPEPTIDASE-A [J].

CHRISTIANSON, DW ;

LIPSCOMB, WN .

ACCOUNTS OF CHEMICAL RESEARCH, 1989, 22 (02) :62-69