Cytokine production by human peripheral blood mononuclear cells: Differential sensitivity to glutamine availability

被引:70
作者
Yaqoob, P [1 ]
Calder, PC [1 ]
机构
[1] Univ Southampton, Inst Human Nutr, Southampton SO16 7PX, Hants, England
关键词
glutamine; cytokine; mononuclear cells; immune system;
D O I
10.1006/cyto.1998.0358
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human peripheral blood mononuclear cells (PBMCs) were cultured in the presence of different glutamine concentrations (0, 0.1, 0.4, 0.6, 2 mM) and stimulated with concanavalin A (Con A) or bacterial lipopolysaccharide (LPS), The concentrations of T lymphocyte- and monocyte-derived cytokines were measured in the culture medium 24 h later, The availability of glutamine significantly increased the production of interleukin (IL)-2 (2-fold), IL-10 (4-fold) and interferon (IFN)-gamma (4.5-fold) by Con A-stimulated PBMCs. Maximal production of these cytokines occurred at 0.1 mM glutamine and increasing the concentration of glutamine above tl;at did not lead to a further increase in cytokine production. Glutamine availability resulted in small increases (24 to 35%) in the production of IL-1 alpha, IL-1 beta, IL-6 and tumour necrosis factor (TNF)-alpha by Con A-stimulated PBMCs; again maximal production occurred at a glutamine concentration of 0.1 mM, Glutamine availability did not influence the production of IL-beta or TNF-alpha by LPS-stimulated PBMCs, while there was a small increase (17 to 32%) in the production of IL-1 alpha, IL-6 and IL-10 by these cells at a glutamine concentration of 0.1 mM. It is concluded that glutamine enhances the production of T lymphocyte-derived cytokines with only minimal effects on production of cytokines by monocytes, (C) 1998 Academic Press.
引用
收藏
页码:790 / 794
页数:5
相关论文
共 42 条
  • [1] GLUTAMINE-METABOLISM IN LYMPHOCYTES OF THE RAT
    ARDAWI, MSM
    NEWSHOLME, EA
    [J]. BIOCHEMICAL JOURNAL, 1983, 212 (03) : 835 - 842
  • [2] GLUTAMINE AND GLUCOSE-METABOLISM IN HUMAN PERIPHERAL LYMPHOCYTES
    ARDAWI, MSM
    [J]. METABOLISM-CLINICAL AND EXPERIMENTAL, 1988, 37 (01): : 99 - 103
  • [3] MUSCLE AND PLASMA AMINO-ACIDS FOLLOWING INJURY - INFLUENCE OF INTERCURRENT INFECTION
    ASKANAZI, J
    CARPENTIER, YA
    MICHELSEN, CB
    ELWYN, DH
    FURST, P
    KANTROWITZ, LR
    GUMP, FE
    KINNEY, JM
    [J]. ANNALS OF SURGERY, 1980, 192 (01) : 78 - 85
  • [4] MUSCLE AND PLASMA AMINO-ACIDS AFTER INJURY - HYPOCALORIC GLUCOSE VS AMINO-ACID INFUSION
    ASKANAZI, J
    FURST, P
    MICHELSEN, CB
    ELWYN, DH
    VINNARS, E
    GUMP, FE
    STINCHFIELD, FE
    KINNEY, JM
    [J]. ANNALS OF SURGERY, 1980, 191 (04) : 465 - 472
  • [5] MUSCLE AND PLASMA AMINO-ACIDS AFTER INJURY - ROLE OF INACTIVITY
    ASKANAZI, J
    ELWYN, DH
    KINNEY, JM
    GUMP, FE
    MICHELSEN, CB
    STINCHFIELD, FE
    FURST, P
    VINNARS, E
    BERGSTROM, J
    [J]. ANNALS OF SURGERY, 1978, 188 (06) : 797 - 803
  • [6] Barclay A.N., 1993, LEUCOCYTE ANTIGEN FA
  • [7] Nitric oxide inhibits the secretion of T-helper 1- and T-helper 2-associated cytokines in activated human T cells
    Bauer, H
    Jung, T
    Tsikas, D
    Stichtenoth, DO
    Frolich, JC
    Neumann, C
    [J]. IMMUNOLOGY, 1997, 90 (02) : 205 - 211
  • [8] METABOLISM OF GLUTAMINE IN LYMPHOCYTES
    BRAND, K
    FEKL, W
    VONHINTZENSTERN, J
    LANGER, K
    LUPPA, P
    SCHOERNER, C
    [J]. METABOLISM-CLINICAL AND EXPERIMENTAL, 1989, 38 (08): : 29 - 33
  • [10] FUEL UTILIZATION BY CELLS OF THE IMMUNE-SYSTEM
    CALDER, PC
    [J]. PROCEEDINGS OF THE NUTRITION SOCIETY, 1995, 54 (01) : 65 - 82