Cytokine production by human peripheral blood mononuclear cells: Differential sensitivity to glutamine availability

被引:70
作者
Yaqoob, P [1 ]
Calder, PC [1 ]
机构
[1] Univ Southampton, Inst Human Nutr, Southampton SO16 7PX, Hants, England
关键词
glutamine; cytokine; mononuclear cells; immune system;
D O I
10.1006/cyto.1998.0358
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Human peripheral blood mononuclear cells (PBMCs) were cultured in the presence of different glutamine concentrations (0, 0.1, 0.4, 0.6, 2 mM) and stimulated with concanavalin A (Con A) or bacterial lipopolysaccharide (LPS), The concentrations of T lymphocyte- and monocyte-derived cytokines were measured in the culture medium 24 h later, The availability of glutamine significantly increased the production of interleukin (IL)-2 (2-fold), IL-10 (4-fold) and interferon (IFN)-gamma (4.5-fold) by Con A-stimulated PBMCs. Maximal production of these cytokines occurred at 0.1 mM glutamine and increasing the concentration of glutamine above tl;at did not lead to a further increase in cytokine production. Glutamine availability resulted in small increases (24 to 35%) in the production of IL-1 alpha, IL-1 beta, IL-6 and tumour necrosis factor (TNF)-alpha by Con A-stimulated PBMCs; again maximal production occurred at a glutamine concentration of 0.1 mM, Glutamine availability did not influence the production of IL-beta or TNF-alpha by LPS-stimulated PBMCs, while there was a small increase (17 to 32%) in the production of IL-1 alpha, IL-6 and IL-10 by these cells at a glutamine concentration of 0.1 mM. It is concluded that glutamine enhances the production of T lymphocyte-derived cytokines with only minimal effects on production of cytokines by monocytes, (C) 1998 Academic Press.
引用
收藏
页码:790 / 794
页数:5
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