Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene tet(X) Variants from Bacterial, Fecal, and Environmental Samples

被引：13

作者：

Fu, Yulin ^{[1
]}

Liu, Dejun ^{[1
]}

Song, Huangwei ^{[1
]}

Liu, Zhihai ^{[1
,2
]}

Jiang, Haiyang ^{[1
]}

Wang, Yang ^{[1
,3
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing, Peoples R China

[2] Qingdao Agr Univ, Coll Chem & Pharmaceut Sci, Qingdao, Peoples R China

[3] China Agr Univ, Coll Vet Med, Beijing Key Lab Detect Technol Anim Derived Food, Beijing, Peoples R China

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2020年 / 64卷 / 04期

基金：

中国国家自然科学基金;

关键词：

tet(X); SYBR green; tigecycline resistance; real-time PCR; TETRACYCLINE RESISTANCE; EMERGENCE; MECHANISM;

D O I：

10.1128/AAC.02292-19

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1) and tet(X2) and high-level tigecycline resistance genes tet(X3), tet(X4), and tet(X5). We showed that the real-time PCR assay developed had high linearity (R-2 >= 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.

引用

页数：6

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