A simple isotope-labeled UHPLC-MS/MS assay for simultaneous quantification of methotrexate, imatinib and dasatinib

被引：1

作者：

Zhang, Yao ^{[1
]}

Liu, Yao ^{[2
]}

Tan, Lu ^{[1
]}

机构：

[1] Southern Med Univ, Affiliated Hosp 3, Dept Pharm, Guangzhou 510630, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 5, Dept Pharm, Zhuhai 519000, Peoples R China

来源：

BIOANALYSIS | 2021年 / 13卷 / 19期

关键词：

dasatinib; imatinib; methotrexate; therapeutic drug monitoring; UHPLC-MS; MS; ACUTE LYMPHOBLASTIC-LEUKEMIA; HIGH-DOSE METHOTREXATE; CHILDREN; PHARMACOKINETICS;

D O I：

10.4155/bio-2021-0162

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: To assist therapeutic dose adjustment in clinic, a reliable concentration measurement is quite necessary for therapeutic drug monitoring. Results: A UHPLC-MS/MS bioassay for simultaneous determination of methotrexate, imatinib and dasatinib using isotope dilution internal standardization has been established and fully validated as per China Food and Drug Administration guideline. After a simple protein precipitation, the analytes were separated by a gradient elution within 3 min and mass detection was performed via ESI+ mode with SRM. The calibration curves were in the range of 5-100 ng/ml for methotrexate, 25-5000 ng/ml for imatinib and 1-250 ng/ml for dasatinib. Imprecision and inaccuracy values were <= 9.44% and <= 12.81% for all analytes, respectively. Conclusion: The developed method is appropriate for therapeutic drug monitoring, being applied to help individualized therapy in patients with acute lymphoblastic leukemia.

引用

页码：1501 / 1510

页数：10

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