Caspase-3: Structure, function, and biotechnological aspects

被引：335

作者：

Asadi, Marzieh ^{[1
]}

Taghizadeh, Saeed ^{[1
]}

Kaviani, Elina ^{[1
]}

Vakili, Omid ^{[2
]}

Taheri-Anganeh, Mortaza ^{[1
]}

Tahamtan, Mahshid ^{[3
]}

Savardashtaki, Amir ^{[1
]}

机构：

[1] Shiraz Univ Med Sci, Dept Med Biotechnol, Sch Adv Med Sci & Technol, Shiraz, Iran

[2] Shiraz Univ Med Sci, Dept Clin Biochem, Sch Pharm & Pharmaceut Sci, Esfahan, Iran

[3] Shiraz Univ Med Sci, Dept Neurosci, Sch Adv Med Sci & Technol, Shiraz, Iran

来源：

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2022年 / 69卷 / 04期

关键词：

apoptosis; biotechnology; caspase-3; diseases; therapeutics; LONG-TERM DEPRESSION; Q-VD-OPH; CELL-DEATH; ALZHEIMERS-DISEASE; MOLECULAR-CLONING; ACTIVE CASPASE-3; GENE-THERAPY; DIFFERENTIATION; ACTIVATION; APOPTOSIS;

D O I：

10.1002/bab.2233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Caspase-3, a cysteine-aspartic acid protease, has recently attracted much attention because of its incredible roles in tissue differentiation, regeneration, and neural development. This enzyme is a key zymogen in cell apoptosis and is not activated until it is cleaved by initiator caspases during apoptotic flux. Since caspase-3 has represented valuable capabilities in the field of medical research, biotechnological aspects of this enzyme, including the production of recombinant type, protein engineering, and designing delivery systems, have been considered as emerging therapeutic strategies in treating the apoptosis-related disorders. To date, several advances have been made in the therapeutic use of caspase-3 in the management of some diseases such as cancers, heart failure, and neurodegenerative disorders. In the current review, we intend to discuss the caspase-3's structure, functions, therapeutic applications, as well as its molecular cloning, protein engineering, and relevant delivery systems.

引用

页码：1633 / 1645

页数：13

共 92 条

[1] Evolution of caspase-mediated cell death and differentiation: twins separated at birth [J].

Bell, Ryan A. V. ;

Megeney, Lynn A. .

CELL DEATH AND DIFFERENTIATION, 2017, 24 (08) :1359-1368

[2]

Cade C E., 2015, Proteases in Apoptosis: Pathways, Protocols and Translational Advances, P31, DOI [DOI 10.1007/978-3-319-19497-4_2, 10.1007/978-3-319-19497-4_2]

[3] Q-VD-OPh, a broad spectrum caspase inhibitor with potent antiapoptotic properties [J].