RFTS-dependent negative regulation of Dnmt1 by nucleosome structure and histone tails

被引：7

作者：

Mishima, Yuichi ^{[1
]}

Brueckner, Laura ^{[1
]}

Takahashi, Saori ^{[1
]}

Kawakami, Toru ^{[2
]}

Arita, Kyohei ^{[3
]}

Oka, Shota ^{[1
]}

Otani, Junji ^{[1
]}

Hojo, Hironobu ^{[2
]}

Shirakawa, Masahiro ^{[4
,5
]}

Shinohara, Akira ^{[6
]}

Watanabe, Mikio ^{[7
]}

Suetake, Isao ^{[1
,5
,7
,8
]}

机构：

[1] Osaka Univ, Inst Prot Res, Lab Epigenet, Suita, Osaka, Japan

[2] Osaka Univ, Inst Prot Res, Lab Organ Chem, Suita, Osaka, Japan

[3] Yokohama City Univ, Grad Sch Nanobiosci, Div Macromol Crystallog, Yokohama, Kanagawa, Japan

[4] Kyoto Univ, Grad Sch Engn, Dept Mol Engn, Kyoto, Japan

[5] Japan Sci & Technol Agcy, CREST, Saitama, Japan

[6] Osaka Univ, Inst Prot Res, Lab Genome Chromosome Funct, Suita, Osaka, Japan

[7] Osaka Univ, Grad Sch Med, Ctr Twin Res, Suita, Osaka, Japan

[8] Koshien Univ, Coll Nutr, Takarazuka, Hyogo, Japan

来源：

FEBS JOURNAL | 2017年 / 284卷 / 20期

关键词：

Dnmt1; histone; modifications; nucleosomes; replication foci targeting sequence; MAINTENANCE DNA METHYLATION; REPLICATION FOCI; METHYLTRANSFERASE; SITES; CHROMATIN; BINDING; DOMAIN; H3; AUTOINHIBITION; PURIFICATION;

D O I：

10.1111/febs.14205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA methylation in promoter regions represses gene expression and is copied over mitotic divisions by Dnmt1. Dnmt1 activity is regulated by its replication foci targeting sequence (RFTS) domain which masks the catalytic pocket. It has been shown that Dnmt1 activity on unmethylated DNA is inhibited in nucleosome cores. In the present study, we aimed to assess the effect of nuclesome formation on maintenance methylation at single CpG resolution. We show that Dnmt1 fully methylates naked linker DNA in dinucleosomes, whereas maintenance methylation was repressed at all CpG sites in nucleosome core particles. Deletion of RFTS partly released obstruction of Dnmt1 activity in core particles. Histone H3 tail peptides inhibited Dnmt1 in an RFTS-dependent manner and repression was modulated by acetylation or methylation. We propose a novel function of RFTS to regulate Dnmt1 activity in nucleosomes.

引用

页码：3455 / 3469

页数：15

共 43 条

[1] The DNA Methyltransferase Dnmt1 Directly Interacts with the SET and RING Finger-associated (SRA) Domain of the Multifunctional Protein Uhrf1 to Facilitate Accession of the Catalytic Center to Hemi-methylated DNA
Berkyurek, Ahmet Can
Suetake, Isao
Arita, Kyohei
Takeshita, Kohei
Nakagawa, Atsushi
Shirakawa, Masahiro
Tajima, Shoji
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (01) : 379 - 386
[2] The DNA methyltransferases of mammals
Bestor, TH
[J]. HUMAN MOLECULAR GENETICS, 2000, 9 (16) : 2395 - 2402
[3] Optimization of baculovirus-mediated expression and purification of hexahistidine-tagged murine DNA (cytosine-C5)-methyltransferase-1 in Spodoptera frugiperda 9 cells
Brank, AS
Van Bemmel, DM
Christman, JK
[J]. PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (01) : 31 - 40
[4] Complete inactivation of DNMT1 leads to mitotic catastrophe in human cancer cells
Chen, Taiping
Hevi, Sarah
Gay, Frederique
Tsujimoto, Naomi
He, Timothy
Zhang, Bailin
Ueda, Yoshihide
Li, En
[J]. NATURE GENETICS, 2007, 39 (03) : 391 - 396
[5] Dnmt1 is essential to maintain progenitors in the perinatal intestinal epithelium
Elliott, Ellen N.
Sheaffer, Karyn L.
Schug, Jonathan
Stappenbeck, Thaddeus S.
Kaestner, Klaus H.
[J]. DEVELOPMENT, 2015, 142 (12): : 2163 - +
[6] DNA methyltransferase Dnmt1 associates with histone deacetylase activity
Fuks, F
Burgers, WA
Brehm, A
Hughes-Davies, L
Kouzarides, T
[J]. NATURE GENETICS, 2000, 24 (01) : 88 - 91
[7] The DNA methyltransferases associate with HP1 and the SUV39H1 histone methyltransferase
Fuks, F
Hurd, PJ
Deplus, R
Kouzarides, T
[J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (09) : 2305 - 2312
[8] Dual Functions of the RFTS Domain of Dnmt1 in Replication-Coupled DNA Methylation and in Protection of the Genome from Aberrant Methylation
Garvilles, Ronald Garingalao
Hasegawa, Takashi
Kimura, Hironobu
Sharif, Jafar
Muto, Masahiro
Koseki, Haruhiko
Takahashi, Saori
Suetake, Isao
Tajima, Shoji
[J]. PLOS ONE, 2015, 10 (09):
[9] De novo methylation of nucleosomal DNA by the mammalian Dnmt1 and Dnmt3A DNA methyltransferases
Gowher, H
Stockdale, CJ
Goyal, R
Ferreira, H
Owen-Hughes, T
Jeltsch, A
[J]. BIOCHEMISTRY, 2005, 44 (29) : 9899 - 9904
[10] Accuracy of DNA methylation pattern preservation by the Dnmt1 methyltransferase
Goyal, R
Reinhardt, R
Jeltsch, A
[J]. NUCLEIC ACIDS RESEARCH, 2006, 34 (04) : 1182 - 1188

← 1 2 3 4 5 →