RAPD analysis of Acanthopanax senticosus genetic diversity

被引：0

作者：

Dai, SL ^{[1
]}

Wang, LX ^{[1
]}

Wu, NH ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Dev Biol, Beijing 100080, Peoples R China

来源：

PROGRESS IN NATURAL SCIENCE | 1998年 / 8卷 / 04期

关键词：

Acanthopanax senticosus; genetic diversity; RAPD analysis; population;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Random amplified polymorphic DNA (RAPD) analysis has been used widely in detecting genetic diversity at population level. A total of 49 individual plants of Acanthopanax senticosus from three populations in Northeast China were randomly sampled. Genomic DNA was extracted and purified from fresh leaves. Intensive RAPD analysis was conducted. The proportions of polymorphic RAPD loci in the three populations were 93.5%, 91.3% and 97.6%, respectively. The average values of genetic diversity of A, senticosus within populations were 0.241, 0.363 and 0.388. The average genetic distances between each population were 0.435, 0.390 and 0.412. These results revealed a high level of genetic diversity of A. senticosus both within and between its natural populations, indicating that it is a flourishing species.

引用

页码：465 / 468

页数：4

共 14 条

[1] AMMON L, 1993, HORTISCIENCE, V28, P1180
[2] EVALUATION OF THE EXTENT OF GENETIC-VARIATION IN MAHOGANIES (MELIACEAE) USING RAPD MARKERS
CHALMERS, KJ
NEWTON, AC
WAUGH, R
WILSON, J
POWELL, W
[J]. THEORETICAL AND APPLIED GENETICS, 1994, 89 (04) : 504 - 508
[3] *DEP BIOL XIAM U, 1978, CHIN FLOR, V54
[4] HARDYS H, 1992, MOL ECOL, P55
[5] *I BOT CHIN AC SCI, 1983, ILL BOOK CHIN HIGH P, V3
[6] GENETIC DISTANCE BETWEEN POPULATIONS
NEI, M
[J]. AMERICAN NATURALIST, 1972, 106 (949) : 283 - +
[7] NEI M, 1978, MOL EVOLUTIONARY GEN
[8] FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS
WELSH, J
MCCLELLAND, M
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (24) : 7213 - 7218
[9] DNA POLYMORPHISMS AMPLIFIED BY ARBITRARY PRIMERS ARE USEFUL AS GENETIC-MARKERS
WILLIAMS, JGK
KUBELIK, AR
LIVAK, KJ
RAFALSKI, JA
TINGEY, SV
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (22) : 6531 - 6535
[10] YAN HJ, 1997, CELL RES, P99

← 1 2 →