Involvement of MicroRNAs in Regulation of Radioresistance of HeLa and DU145 Cells

被引：1

作者：

Chebotarev, D. A. ^{[1
]}

Makhotkin, M. A. ^{[1
]}

Naboka, A. V. ^{[1
]}

Tyutyakina, M. G. ^{[1
]}

Cherkasova, E. N. ^{[1
]}

Tarasov, V. A. ^{[1
]}

机构：

[1] Russian Acad Sci, Southern Sci Ctr, Rostov Na Donu 344006, Russia

来源：

RUSSIAN JOURNAL OF GENETICS | 2019年 / 55卷 / 09期

关键词：

microRNA; massively parallel sequencing; radioresistance; apoptosis; cell cycle; cancer; DU145; HeLa; TUMOR-SUPPRESSOR PTEN; DNA-DAMAGE; TARGETING PTEN; PROTEIN; EXPRESSION; CARCINOMA; RADIATION; REPAIR; RADIOSENSITIVITY; METHYLATION;

D O I：

10.1134/S1022795419090047

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Using the method of massively parallel sequencing, differential miRNA expression profiles in the original and radioresistant variants of HeLa and DU145 cell lines after radiation exposure at the dose of 4 Gy were obtained. Comparative analysis made it possible to identify a group of miRNAs aberrant expression of which reflected the changes in cell metabolism of radioresistant variants that remained across a number of cell generations as compared to the original ones. The obtained results suggest that the role of miRNAs is associated with the maintenance of functional interaction between DNA methyltransferases, Myc transcription factor, and PTEN phosphatase in regulating the activity of PI3K/AKT and MAPK/ERK signaling pathways. This may underlie differences in realization of radioresistance of HeLa and DU145 cells.

引用

页码：1072 / 1081

页数：10

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