Maintenance of telomeres in SV40-transformed pre-immortal and immortal human fibroblasts

被引:0
作者
Small, MB
Hubbard, K
Pardinas, JR
Marcus, AM
Dhanaraj, SN
Sethi, KA
机构
[1] UNIV MED & DENT NEW JERSEY, NEW JERSEY MED SCH, DEPT MICROBIOL & MOL GENET, NEWARK, NJ 07103 USA
[2] CUNY CITY COLL, DEPT BIOL, NEW YORK, NY 10031 USA
关键词
D O I
10.1002/(SICI)1097-4652(199609)168:3<727::AID-JCP26>3.0.CO;2-U
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Shortening of telomeres has been hypothesized to contribute to cellular senescence and may play a role in carcinogenesis of human cells. Furthermore, activation of telomerase has frequently been demonstrated in tumor-derived and in vitro immortalized cells. In this study, we have assessed these phenomena during the life span of simian virus 40 (SV40)-transformed preimmortal and immortal human fibroblasts. We observed progressive reduction in telomere length in preimmortal transformed cells with extended proliferative capacity, with the most dramatic shortening at late passage. Telomere lengths became stabilized (or increased) in immortal fibroblasts accompanied, in one case, by the activation of telomerase. However, an independent immortal cell line that displayed stable telomeres did not have detectable telomerase activity. Furthermore, we found significant telomerase activity in two preimmortal derivatives. Our results provide further evidence for maintenance of telomeres in immortalized human fibroblasts, but they suggest a lack of causal relationship between telomerase activation and immortalization. (C) 1996 Wiley-Liss, Inc.
引用
收藏
页码:727 / 736
页数:10
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