Molecular Cloning and Expression Analysis of a Delta 6-Fatty Acid Desaturase Gene from Rhizopus stolonifer Strain YF6 Which Can Accumulate High Levels of Gamma-Linolenic Acid

被引：10

作者：

Wan, Xia ^{[1
,2
]}

Zhang, Yinbo ^{[1
]}

Wang, Ping ^{[1
]}

Jiang, Mulan ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China

[2] Minist Agr, Key Lab Oil Crop Biol, Wuhan 430062, Peoples R China

来源：

JOURNAL OF MICROBIOLOGY | 2011年 / 49卷 / 01期

基金：

中国国家自然科学基金;

关键词：

desaturase; Rhizopus stolonifer; gamma-linolenic acid; FATTY-ACID; CUNNINGHAMELLA-ECHINULATA; HETEROLOGOUS EXPRESSION; MORTIERELLA FUNGUS; DELTA(6)-DESATURASE; IDENTIFICATION; METABOLISM; HEALTH;

D O I：

10.1007/s12275-011-0254-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Delta(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22.4% of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.

引用

页码：151 / 154

页数：4

共 21 条

[1] Production of γ-linolenic acid in Lotus japonicus and Vigna angularis by expression of the Δ6-fatty-acid desaturase gene isolated from Mortierella alpina [J].

Chen, R ;

Tsuda, S ;

Matsui, K ;

Fukuchi-Mizutani, M ;

Ochiai, M ;

Shimizu, S ;

Sakuradani, E ;

Aoki, T ;

Imaizumi, R ;

Ayabe, S ;

Tanaka, Y .

PLANT SCIENCE, 2005, 169 (03) :599-605

[2] Importance of dietary γ-linolenic acid in human health and nutrition? [J].

Fan, YY ;

Chapkin, RS .

JOURNAL OF NUTRITION, 1998, 128 (09) :1411-1414

[3] Role of stearoyl-coenzyme A desaturase in regulating lipid metabolism [J].

Flowers, Matthew T. ;

Ntambi, James M. .

CURRENT OPINION IN LIPIDOLOGY, 2008, 19 (03) :248-256

[4] Production of γ-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel [J].

Gema, H ;

Kavadia, A ;

Dimou, D ;

Tsagou, V ;

Komaitis, M ;

Aggelis, G .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 58 (03) :303-307

[5] High-level production of γ-linolenic acid in Brassica juncea using a Δ6 desaturase from Pythium irregulare [J].

Hong, H ;

Datla, N ;

Reed, DW ;

Covello, PS ;

MacKenzie, SL ;

Qiu, X .

PLANT PHYSIOLOGY, 2002, 129 (01) :354-362

[6] NUTRITIONAL AND MEDICAL IMPORTANCE OF GAMMA-LINOLENIC ACID [J].

HORROBIN, DF .

PROGRESS IN LIPID RESEARCH, 1992, 31 (02) :163-194

[7]

HORROBIN DF, 1993, AM J CLIN NUTR, V57, P732

[8] Gamma linolenic acid: An antiinflammatory omega-6 fatty acid [J].

Kapoor, Rakesh ;

Huang, Yung-Sheng .

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2006, 7 (06) :531-534

[9] Δ6-Desaturase of Mucor rouxii with high similarity to plant Δ6-desaturase and its heterologous expression in Saccharomyces cerevisiae [J].

Laoteng, K ;

Mannontarat, R ;

Tanticharoen, M ;

Cheevadhanarak, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 279 (01) :17-22

[10] Identification and characterization of a novel a†6-fatty acid desaturase gene from Rhizopus nigricans [J].

Lu, He ;

Li, Jia-Na ;

Chai, You-Rong ;

Zhang, Xue-Kun .

MOLECULAR BIOLOGY REPORTS, 2009, 36 (08) :2291-2297

← 1 2 3 →