TRPC5 is a regulator of hippocampal neurite length and growth cone morphology

被引：300

作者：

Greka, A

Navarro, B

Oancea, E

Duggan, A

Clapham, DE

机构：

[1] Childrens Hosp, Howard Hughes Med Inst, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Boston, MA 02115 USA

[3] Harvard MIT Div Hlth Sci & Technol, Boston, MA 02115 USA

[4] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA

来源：

NATURE NEUROSCIENCE | 2003年 / 6卷 / 08期

关键词：

D O I：

10.1038/nn1092

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Growth cone motility is regulated by both fast voltage-dependent Ca2+ channels and by unknown receptor-operated Ca2+ entry mechanisms. Transient receptor potential (TRP) homomeric TRPC5 ion channels are receptor-operated, Ca2+-permeable channels predominantly expressed in the brain. Here we show that TRPC5 is expressed in growth cones of young rat hippocampal neurons. Our results indicate that TRPC5 channel subunits interact with the growth cone-enriched protein stathmin 2, are packaged into vesicles and are carried to newly forming growth cones and synapses. Once in the growth cone, TRPC5 channels regulate neurite extension and growth-cone morphology. Dominant-negative TRPC5 expression allowed significantly longer neurites and filopodia to form. We conclude that TRPC5 channels are important components of the mechanism controlling neurite extension and growth cone morphology.

引用

页码：837 / 845

页数：9