Cellular imagery with total internal reflection holographic microscopy

被引：0

作者：

Ash, William M., III ^{[1
]}

Kim, Myung K. ^{[1
]}

机构：

[1] Univ S Florida, Dept Phys, Digital Holog & Microscopy Lab, Tampa, FL 33620 USA

来源：

IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES VII | 2009年 / 7182卷

关键词：

Evanescent; digital holography; TIR; motility; surface phase profile; tissue; membrane;

D O I：

10.1117/12.808029

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Evanescent wave phase profiling is incorporated with digital holography into a new surface imaging technique termed total internal reflection holographic microscopy (TIRHM). Quantitative images of tissue structures and profiles of cellular membranes are presented to demonstrate the method's performance capabilities. Applications of this technique include measurements of cellular membranes and their transport processes without the addition of fluorophores. The angular spectrum method to compensate for tilt anamorphism due to the inclined TIR plane is also discussed.

引用

页数：8

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