The Anti-Inflammatory Effects of Angiogenin in an Endotoxin Induced Uveitis in Rats

被引：28

作者：

Park, Jihae ^{[1
]}

Kim, Jee Taek ^{[2
]}

Lee, Soo Jin ^{[1
]}

Kim, Jae Chan ^{[1
,2
]}

机构：

[1] Cheil Eye Hosp, Cheil Eye Res Inst, Dept Ophthalmol, Daegu 8032, South Korea

[2] Chung Ang Univ Hosp, Coll Med, Dept Ophthalmol, Seoul 2241, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2020年 / 21卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

angiogenin; uveitis; EIU rat model; NF-KAPPA-B; DOWN-REGULATION; CANCER; IL-10; INFLAMMATION; MECHANISMS; PROTEIN; EYE; PHOSPHORYLATION; ACTIVATION;

D O I：

10.3390/ijms21020413

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Angiogenin (ANG) is involved in the innate immune system and inflammatory disease. The aim of this study is to evaluate the anti-inflammatory effects of ANG in an endotoxin induced uveitis (EIU) rat model and the pathways involved. EIU rats were treated with balanced salt solution (BSS), a non-functional mutant ANG (mANG), or wild-type ANG (ANG). The integrity of the blood-aqueous barrier was evaluated by the infiltrating cell and protein concentrations in aqueous humor. Histopathology, Western blot, and real-time qRT-PCR of aqueous humor and ocular tissue were performed to analyze inflammatory cytokines and transcription factors. EIU treated with ANG had decreased inflammatory cells and protein concentrations in the anterior chamber. Compared to BSS and mANG, ANG treatment showed reduced expression of IL-1 beta, IL-8, TNF-alpha, and Myd88, while the expression of IL-4 and IL-10 was increased. Western blot of ANG treatment showed decreased expression of IL-6, inducible nitric oxide synthase (iNOS), IL-1 beta, TNF-alpha, and phosphorylated NF-kappa B and increased expression of IL-10. In conclusion, ANG seems to reduce effectively immune mediated inflammation in the EIU rat model by reducing the expression of proinflammatory cytokines, while increasing the expression of anti-inflammatory cytokines through pathways related to NF-kappa B. Therefore, ANG shows potential for effectively suppressing immune-inflammatory responses in vivo.

引用

页数：14