Rational design of memory in eukaryotic cells

被引：171
作者：
Ajo-Franklin, Caroline M.
Drubin, David A.
Eskin, Julian A.
Gee, Elaine P. S.
Landgraf, Dirk
Phillips, Ira
Silver, Pamela A. [1 ]
机构：
[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA
[2] Harvard Univ, MIT, Program Biophys, Cambridge, MA 02139 USA
来源：
GENES & DEVELOPMENT | 2007年 / 21卷 / 18期
关键词：
rational design; synthetic biology; positive feedback; memory; in vivo quantitation;
D O I：
10.1101/gad.1586107
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The ability to logically engineer novel cellular functions promises a deeper understanding of biological systems. Here we demonstrate the rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback. We built a set of transcriptional activators and quantitatively characterized their effects on gene expression in living cells. Modeling in conjunction with the quantitative characterization of the activator-promoter pairs accurately predicts the behavior of the memory network. This study demonstrates the power of taking advantage of components with measured quantitative parameters to specify eukaryotic regulatory networks with desired properties.
引用
收藏
页码：2271 / 2276
页数：6
相关论文
共 34 条
  • [1] Alon U., 2006, INTRO SYSTEMS BIOL D
  • [2] Synthetic biology: new engineering rules for an emerging discipline
    Andrianantoandro, Ernesto
    Basu, Subhayu
    Karig, David K.
    Weiss, Ron
    [J]. MOLECULAR SYSTEMS BIOLOGY, 2006, 2 (1) : 2006.0028
  • [3] Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli
    Atkinson, MR
    Savageau, MA
    Myers, JT
    Ninfa, AJ
    [J]. CELL, 2003, 113 (05) : 597 - 607
  • [4] Positive feedback in eukaryotic gene networks:: cell differentiation by graded to binary response conversion
    Becskei, A
    Séraphin, B
    Serrano, L
    [J]. EMBO JOURNAL, 2001, 20 (10) : 2528 - 2535
  • [5] Toward controlling gene expression at will:: Specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks
    Beerli, RR
    Segal, DJ
    Dreier, B
    Barbas, CF
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) : 14628 - 14633
  • [6] THE SEGMENT-SPECIFIC GENE KROX-20 ENCODES A TRANSCRIPTION FACTOR WITH BINDING-SITES IN THE PROMOTER REGION OF THE HOX-1.4 GENE
    CHAVRIER, P
    VESQUE, C
    GALLIOT, B
    VIGNERON, M
    DOLLE, P
    DUBOULE, D
    CHARNAY, P
    [J]. EMBO JOURNAL, 1990, 9 (04) : 1209 - 1218
  • [7] Designing biological systems
    Drubin, David A.
    Way, Jeffrey C.
    Silver, Pamela A.
    [J]. GENES & DEVELOPMENT, 2007, 21 (03) : 242 - 254
  • [8] Construction of a genetic toggle switch in Escherichia coli
    Gardner, TS
    Cantor, CR
    Collins, JJ
    [J]. NATURE, 2000, 403 (6767) : 339 - 342
  • [9] Single-cell quantification of molecules and rates using open-source microscope-based cytometry
    Gordon, Andrew
    Colman-Lerner, Alejandro
    Chin, Tina E.
    Benjamin, Kirsten R.
    Yu, Richard C.
    Brent, Roger
    [J]. NATURE METHODS, 2007, 4 (02) : 175 - 181
  • [10] A bottom-up approach to gene regulation
    Guido, NJ
    Wang, X
    Adalsteinsson, D
    McMillen, D
    Hasty, J
    Cantor, CR
    Elston, TC
    Collins, JJ
    [J]. NATURE, 2006, 439 (7078) : 856 - 860
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