Function of Metallothionein-3 in Neuronal Cells: Do Metal Ions Alter Expression Levels of MT3?

被引：23

作者：

Bousleiman, Jamie ^{[1
]}

Pinsky, Alexa ^{[1
]}

Ki, Sohee ^{[1
]}

Su, Angela ^{[1
]}

Morozova, Irina ^{[2
]}

Kalachikov, Sergey ^{[2
]}

Wiqas, Amen ^{[3
]}

Silver, Rae ^{[4
,5
,6
,7
]}

Sever, Mary ^{[1
]}

Austin, Rachel Narehood ^{[1
]}

机构：

[1] Columbia Univ, Barnard Coll, Dept Chem, New York, NY 10027 USA

[2] Columbia Univ, Dept Chem Engn, Ctr Genome Technol & Biomol Engn, New York, NY 10027 USA

[3] Columbia Univ, Barnard Coll, Dept Biol, New York, NY 10027 USA

[4] Columbia Univ, Barnard Coll, Dept Psychol, New York, NY 10027 USA

[5] Columbia Univ, Barnard Coll, Program Neurosci, New York, NY 10027 USA

[6] Columbia Univ, Dept Psychol, New York, NY 10027 USA

[7] Columbia Hlth Sci, Dept Pathol & Cell Biol, New York, NY 10027 USA

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 06期

基金：

美国国家科学基金会;

关键词：

metallothionein; MT3; metalloneurochemistry; lead neurotoxicity; gene expression; microarrays; dentate gyrus; GROWTH-INHIBITORY FACTOR; GENE-EXPRESSION; ALZHEIMERS-DISEASE; MESSENGER-RNA; SELENOPROTEIN-W; HEAVY-METALS; BINDING; BRAIN; ZINC; ACTIVATION;

D O I：

10.3390/ijms18061133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A study of factors proposed to affect metallothionein-3 (MT3) function was carried out to elucidate the opaque role MT3 plays in human metalloneurochemistry. Gene expression of Mt2 and Mt3 was examined in tissues extracted from the dentate gyrus of mouse brains and in human neuronal cell cultures. The whole-genome gene expression analysis identified significant variations in the mRNA levels of genes associated with zinc homeostasis, including Mt2 and Mt3. Mt3 was found to be the most differentially expressed gene in the identified groups, pointing to the existence of a factor, not yet identified, that differentially controls Mt3 expression. To examine the expression of the human metallothioneins in neurons, mRNA levels of MT3 and MT2 were compared in BE(2)C and SH-SY5Y cell cultures treated with lead, zinc, cobalt, and lithium. MT2 was highly upregulated by Zn2+ in both cell cultures, while MT3 was not affected, and no other metal had an effect on either MT2 or MT3.

引用

页数：17

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