Spectrofluorimetric and micelle-enhanced spectrofluorimetric determination of gatifloxacin in human urine and serum

A spectrofluorimetric method to determine gatifloxacin has been developed and applied to the quantification of this fluoroquinolone in spiked human urine and serum. The native fluorescence of gatifloxacin allow the determination of 0.040-0.700 mug mL(-1) of this molecule in aqueous solution containing acetic acid-sodium acetate buffer (pH 3.5), with lambda(exc) = 292 nm and lambda(em) = 484 nm. Micelle-enhanced fluorescence led to 75% higher analytical signals in presence of 12 mM sodium dodecyl sulphate, which allow the determination of 0.020-0.450 mug mL(-1) fluoroquinolone with 292 nm and 470 nm. Both methods were successfully applied to gatifloxacin determination in spiked human urine and serum. (C) 2004 Elsevier B.V. All rights reserved.