Dissociation of superoxide production by mitochondrial complex I from NAD(P)H redox state

被引：29
作者：
Lambert, Adrian J. [1 ]
Buckingham, Julie A. [1 ]
Brand, Martin D. [1 ]
机构：
[1] MRC, Dunn Human Nutr Unit, Cambridge CB2 0XY, England
来源：
FEBS LETTERS | 2008年 / 582卷 / 12期
基金：
英国医学研究理事会; 英国惠康基金;
关键词：
NADH : ubiquinone oxidoreductase; mitochondria; superoxide; NAD(P)H; hydrogen peroxide; reactive oxygen species;
D O I：
10.1016/j.febslet.2008.04.030
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The relationship between the rate of superoxide production by complex I and NAD(P) H redox state was investigated in rat skeletal muscle mitochondria. A high rate of superoxide production was observed during succinate oxidation; the rate during pyruvate oxidation was over fourfold lower. However, the NAD(P) H pool was significantly less reduced during succinate oxidation than during pyruvate oxidation. We conclude that there is no unique relationship between superoxide production by complex I and the reduction state of the NAD(P) H pool. Our data suggest that less than 10% of the superoxide originates from the flavin site during reverse electron transport from succinate. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：1711 / 1714
页数：4
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