Virucidal Action Against Avian Influenza H5N1 Virus and Immunomodulatory Effects of Nanoformulations Consisting of Mesoporous Silica Nanoparticles Loaded with Natural Prodrugs

被引：28

作者：

AbouAitah, Khaled ^{[1
,2
]}

Swiderska-Sroda, Anna ^{[1
]}

Kandeil, Ahmed ^{[3
]}

Salman, Asmaa ^{[4
]}

Wojnarowicz, Jacek ^{[1
]}

Ali, Mohamed A. ^{[3
]}

Opalinska, Agnieszka ^{[1
]}

Gierlotka, Stanislaw ^{[1
]}

Ciach, Tomasz ^{[5
]}

Lojkowski, Witold ^{[1
]}

机构：

[1] Polish Acad Sci, Inst High Pressure Phys, Lab Nanostruct & Nanomed, Warsaw, Poland

[2] Natl Res Ctr NRC, Pharmaceut & Drug Ind Res Div, Med & Aromat Plants Res Dept, Giza 12622, Egypt

[3] Natl Res Ctr NRC, Environm Res Div, Water Pollut Res Dept, Ctr Sci Excellence Influenza Viruses, Giza 12622, Egypt

[4] Natl Res Ctr NRC, Pharmaceut & Drug Ind Res Div, Med & Pharmaceut Chem Dept, Giza 12622, Egypt

[5] Warsaw Univ Technol, Fac Chem & Proc Engn, Biomed Engn Lab, Warsaw, Poland

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2020年 / 15卷

关键词：

virucidal action; influenza H5N1 virus; immunomodulatory and anti-inflammatory; nanoformulations-drug delivery system; shikimic acid and quercetin natural prodrugs; mesoporous silica nanoparticles; NF-KAPPA-B; A VIRUS; SILVER NANOPARTICLES; DRUG-DELIVERY; SURFACE FUNCTIONALIZATION; CYTOKINE RESPONSES; ANTIVIRAL ACTIVITY; ENTRY INHIBITORS; SHIKIMIC ACID; NITRIC-OXIDE;

D O I：

10.2147/IJN.S247692

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Background: Combating infectious diseases caused by influenza virus is a major challenge due to its resistance to available drugs and vaccines, side effects, and cost of treatment. Nanomedicines are being developed to allow targeted delivery of drugs to attack specific cells or viruses. Materials and Methods: In this study, mesoporous silica nanoparticles (MSNs) functionalized with amino groups and loaded with natural prodrugs of shikimic acid (SH), quercetin (QR) or both were explored as a novel antiviral nanoformulations targeting the highly pathogenic avian influenza H5N1 virus. Also, the immunomodulatory effects were investigated in vitro tests and anti-inflammatory activity was determined in vivo using the acute carrageenan-induced paw edema rat model. Results: Prodrugs alone or the MSNs displayed weaker antiviral effects as evidenced by virus titers and plaque formation compared to nanoformulations. The MSNs-NH2-SH and MSNs-NH2-SH-QR2 nanoformulations displayed a strong virucidal by inactivating the H5N1 virus. They induced also strong immunomodulatory effects: they inhibited cytokines (TNF-alpha, IL-1 beta) and nitric oxide production by approximately 50% for MSNs-NH2-SH-QR(2) (containing both SH and QR). Remarkable anti-inflammatory effects were observed during in vivo tests in an acute carrageenan-induced rat model. Conclusion: Our preliminary findings show the potential of nanotechnology for the application of natural prodrug substances to produce a novel safe, effective, and affordable antiviral drug.

引用

页码：5181 / 5202

页数：22

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