Characterization of Circulating Endothelial Cells in Acute Myocardial Infarction

被引：61

作者：

Damani, Samir ^{[1
]}

Bacconi, Andrea ^{[2
]}

Libiger, Ondrej ^{[1
,2
]}

Chourasia, Aparajita H. ^{[3
]}

Serry, Rod ^{[4
]}

Gollapudi, Raghava ^{[5
,6
]}

Goldberg, Ron ^{[5
,6
]}

Rapeport, Kevin ^{[5
,6
]}

Haaser, Sharon ^{[1
]}

Topol, Sarah ^{[1
]}

Knowlton, Sharen ^{[1
]}

Bethel, Kelly ^{[2
]}

Kuhn, Peter ^{[2
]}

Wood, Malcolm ^{[2
]}

Carragher, Bridget ^{[2
]}

Schork, Nicholas J. ^{[1
,2
]}

Jiang, John ^{[3
]}

Rao, Chandra ^{[3
]}

Connelly, Mark ^{[3
]}

Fowler, Velia M. ^{[2
]}

Topol, Eric J. ^{[1
,2
]}

机构：

[1] Scripps Translat Sci Inst, La Jolla, CA 92037 USA

[2] Scripps Res Inst, La Jolla, CA 92037 USA

[3] Veridex LLC, Huntingdon Valley, PA 19006 USA

[4] Palomar Pomerado Hlth, Escondido, CA 92025 USA

[5] Sharp Mem Hosp & Rehabil Ctr, San Diego, CA 92123 USA

[6] Grossmont Hosp, San Diego, CA 92123 USA

来源：

SCIENCE TRANSLATIONAL MEDICINE | 2012年 / 4卷 / 126期

关键词：

DISEASE; ATHEROSCLEROSIS; RISK;

D O I：

10.1126/scitranslmed.3003451

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Acute myocardial infarction (MI), which involves the rupture of existing atheromatous plaque, remains highly unpredictable despite recent advances in the diagnosis and treatment of coronary artery disease. Accordingly, a clinical measurement that can predict an impending MI is desperately needed. Here, we characterize circulating endothelial cells (CECs) using an automated and clinically feasible CEC three-channel fluorescence microscopy assay in 50 consecutive patients with ST-segment elevation MI and 44 consecutive healthy controls. CEC counts were significantly elevated in MI cases versus controls, with median numbers of 19 and 4 cells/ml, respectively (P = 1.1 x 10(-10)). A receiver-operating characteristic (ROC) curve analysis demonstrated an area under the ROC curve of 0.95, suggesting near-dichotomization of MI cases versus controls. We observed no correlation between CECs and typical markers of myocardial necrosis (rho = 0.02, creatine kinase-myocardial band; r = -0.03, troponin). Morphological analysis of the microscopy images of CECs revealed a 2.5-fold increase (P < 0.0001) in cellular area and a twofold increase (P < 0.0001) in nuclear area of MI CECs versus healthy controls, age-matched CECs, as well as CECs obtained from patients with preexisting peripheral vascular disease. The distribution of CEC images that contained from 2 to 10 nuclei demonstrates that MI patients were the only subject group to contain more than 3 nuclei per image, indicating that multicellular and multinuclear clusters are specific for acute MI. These data indicate that CEC counts may serve as a promising clinical measure for the prediction of atherosclerotic plaque rupture events.

引用

页数：9

共 20 条

[1] Relationship between circulating endothelial cells and the predicted risk of cardiovascular events in acute coronary syndromes [J].