Indole-3-carbinol alleviates carbon tetrachloride-induced liver injury by inhibiting inflammatory response and regulating lipid metabolism

被引:9
作者
Ahn, Meejung [1 ,2 ]
Kim, Jeongtae [1 ,3 ]
Yang, Dawun [4 ]
Chun, Ji-Yeon [5 ]
Kim, Gi Ok [4 ]
Shin, Taekyun [1 ]
机构
[1] Jeju Natl Univ, Coll Vet Med, Dept Vet Anat, Jeju 63243, South Korea
[2] Sangji Univ, Coll Life Sci, Dept Anim Sci, Wonju 26339, South Korea
[3] Kosin Univ, Dept Anat, Coll Med, Busan 49267, South Korea
[4] Jeju Biodivers Res Inst, Jeju Technopk, Jeju 63608, South Korea
[5] Jeju Natl Univ, Coll Engn, Dept Food Bioengn, Jeju 63243, South Korea
关键词
Indole-3-carbinol; Carbon tetrachloride; Inflammation; Adipocyte differentiation; ADIPOCYTE DIFFERENTIATION; ADIPOSE-TISSUE; STELLATE CELLS; KUPFFER CELLS; ADIPOGENESIS; ANTIOXIDANT; OBESITY; MOUSE;
D O I
10.1007/s13596-020-00452-8
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The hepatoprotective effects of indole-3-carbinol (I3C), one of the byproducts of the breakdown of glucosinolate glucobrassicin, were examined in mice with carbon tetrachloride (CCl4)-induced hepatotoxicity. I3C was administered orally at a dose of 50 or 100 mg/kg to C57BL/6 mice for 3 consecutive days with or without a subsequent single oral administration of CCl4 (1.5 mL/kg). I3C treatment significantly suppressed the increases in alanine aminotransferase and aspartate aminotransferase levels in the sera of CCl4-injured mice. In addition, I3C treatment markedly suppressed CCl4-induced hepatic inflammation, by suppressing the activation of macrophages and Kupffer cells in the liver. To examine the effects of I3C on lipid accumulation, 3T3-L1 pre-adipocytes were differentiated with I3C or vehicle control. I3C treatment reduced lipid accumulation, which appeared to be associated with the downregulation of adipogenic transcription factors, such as CCAAT/enhancer-binding protein alpha, peroxisome proliferator-activated receptor gamma, and lipid accumulation-related genes, including adipocyte protein-2. Taken together, the present study suggest that I3C treatment reduces hepatocyte death and lipid accumulation in hepatocytes in CCl4-induced hepatic injury with concurrent suppression of macrophages/Kupffer cells, which was further evidenced by I3C-treated pre-adipocytes in vitro, through downregulation of lipid metabolism.
引用
收藏
页码:371 / 378
页数:8
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