RGDC Peptide Modified Quantum Dots Labelling and Imaging of Tumor Cells

被引：0

作者：

Guo Yi ^{[2
]}

Li Chun-rong ^{[2
]}

Shen Huai-bin ^{[1
]}

Zhang Xue-zhong ^{[2
]}

Li Lin-song ^{[1
]}

Yu Qian ^{[3
]}

Xu Li ^{[2
]}

机构：

[1] Henan Univ, Key Lab Special Funct Mat, Minist Educ, Kaifeng 475004, Peoples R China

[2] Jilin Univ, Coll Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China

[3] Jilin Univ, Dept Drug Adm, China Japan Union Hosp, Changchun 130033, Peoples R China

来源：

CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2011年 / 27卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Arginine-glycine-aspartic acid-cysteine(RGDC) peptide; Quantum dot; Fluorescence labelling; LIVING CELLS; LIVE CELLS; NANOCRYSTALS; TRACKING;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The labelling and imaging of tumor cells were investigated via arginine-glycine-aspartic acid-cysteine(RGDC) peptide-labelled quantum dots(QDs). The results show that RGDC modified QDs can label SMMC-7721 tumor cells and adhere to cellular membrane. In constrast, the unmodified QDs are mainly dispersed around the cell. We also found that the RGDC-QDs can penetrate into the cell at 2 h of incubation. After 6 h of incubation, RGDC-QDs can accumulate in a unique intracellular region.

引用

页码：832 / 835

页数：4

共 27 条

[1] Quantum dot-insect neuropeptide conjugates for fluorescence imaging, transfection, and nucleus targeting of living cells [J].

Biju, Vasudevanpillai ;

Muraleedharan, Damodaran ;

Nakayama, Ken-ichi ;

Shinohara, Yasuo ;

Itoh, Tamitake ;

Baba, Yoshinobu ;

Ishikawa, Mitsuru .

LANGMUIR, 2007, 23 (20) :10254-10261

[2] Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells [J].

Cambi, Alessandra ;

Lidke, Diane S. ;

Arndt-Jovin, Donna J. ;

Figdor, Carl G. ;

Jovin, Thomas M. .

NANO LETTERS, 2007, 7 (04) :970-977

[3] Synthesis and Characterization of Near-Infrared Cu-In-Se/ZnS Core/Shell Quantum Dots for In vivo Imaging [J].

Cassette, E. ;

Pons, T. ;

Bouet, C. ;

Helle, M. ;

Bezdetnaya, L. ;

Marchal, F. ;

Dubertret, B. .

CHEMISTRY OF MATERIALS, 2010, 22 (22) :6117-6124

[4] Cholera toxin B conjugated quantum dots for live cell Labeling [J].

Chakraborty, Subhasish K. ;

Fitzpatrick, James A. J. ;

Phillippi, Julie A. ;

Andreko, Susan ;

Waggoner, Alan S. ;

Bruchez, Marcel P. ;

Ballou, Byron .

NANO LETTERS, 2007, 7 (09) :2618-2626

[5] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[6] Long-term exposure to CdTe quantum dots causes functional impairments in live cells [J].

Cho, Sung Ju ;

Maysinger, Dusica ;

Jain, Manasi ;

Roder, Beate ;

Hackbarth, Steffen ;

Winnik, Francoise M. .

LANGMUIR, 2007, 23 (04) :1974-1980

[7] Cell-penetrating quantum dots based on multivalent and endosome-disrupting surface coatings [J].

Duan, Hongwei ;

Nie, Shuming .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (11) :3333-3338

[8] Direct immunotluorescence of plant Microtubules based on semiconductor nanocrystals [J].

Eggenberger, K. ;

Merkulov, A. ;

Darbandi, M. ;

Nann, T. ;

Nick, P. .

BIOCONJUGATE CHEMISTRY, 2007, 18 (06) :1879-1886

[9] Synthesis of a precursor dipeptide of RGDS (Arg-Gly-Asp-Ser) catalysed by the industrial protease alcalase [J].

Hou, Rui-Zhen ;

Yang, Yan ;

Li, Gang ;

Huang, Yi-Bing ;

Wang, Hua ;

Liu, Yun-Jia ;

Xu, Li ;

Zhang, Xue-Zhong .

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2006, 44 (73-80) :73-80

[10] Plasmonic nanosensors for imaging intracellular biomarkers in live cells [J].

Kumar, Sonia ;

Harrison, Nathan ;

Richards-Kortum, Rebecca ;

Sokolov, Konstantin .

NANO LETTERS, 2007, 7 (05) :1338-1343

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