Induction of mucosal and systemic immune response by oral immunization with H-pylori lysates encapsulated in poly(D,L-lactide-co-glycolide) microparticles

Helicobacter pylori is a major cause of chronic antral gastritis and peptic ulcer diseases. Several kinds of poly(D,L-lactide-co-glycolide) microparticles containing H. pylori whole-cell lysate (PLG-HP) were prepared by the solvent evaporation method using double emulsion. Physical properties, such as particle size, protein content, and morphology were investigated. All prepared microparticles showed a smooth surface morphology from 0.5-0.86 mu m in diameter and high degree of encapsulation efficiency from 62-75%. SDS-PAGE and immunoblotting of extracted antigen confirmed that the molecular weight and antigenicity of the antigen remained unaltered by the encapsulation procedure. Following the oral immunization of the microparticles to mice, antibody production was assayed in serum and gut washings by ELISA and antibody secreting cells were determined in intestinal lamina propria lymphocytes (LPL) by ELISPOT. Multiple oral immunizations induced significant H. pylori-specific intestinal IgA response as well as serum IgG response than those detected with soluble antigen (P < 0.001). The presence of antibody-secreting cell in intestinal lamina propria lymphocytes (LPL) was correlated with IgA level in gut washing fluids. After boosting at week-8, the antibody induction levels were highly increased irrespective of microparticles prepared with different PLG molecular weights. These data suggested that PLG-HP could stimulate the H. pylori-specific mucosal and systemic response in vivo and might be useful adjuvant in future H. pylori vaccine development. (C) 1999 Elsevier Science Ltd. All rights reserved.