Expression of latent and replicative-infection genes of Epstein-Barr virus in macrophage

被引：34

作者：

Shimakage, M

Kimura, M

Yanoma, S

Ibe, M

Yokota, S

Tsujino, G

Kozuka, T

Dezawa, T

Tamura, S

Ohshima, A

Yutsudo, M

Hakura, A

机构：

[1] Osaka Natl Hosp, Clin Res Inst, Chuo Ku, Osaka 5400006, Japan

[2] Osaka Natl Hosp, Dept Pediat, Osaka, Japan

[3] Osaka Natl Hosp, Dept Dermatol, Osaka, Japan

[4] Kansai Shinkyu Coll, Dept Anat, Kumatori, Osaka, Japan

[5] Kanagawa Canc Ctr, Clin Res Inst, Yokohama, Kanagawa, Japan

[6] Yokohama City Univ, Sch Med, Dept Pediat, Yokohama, Kanagawa, Japan

[7] Wakayama Med Coll, Dept Pediat, Wakayama, Japan

[8] Wakayama Med Coll, Dept Otorhinolaryngol, Wakayama, Japan

[9] Wakayama Med Coll, Dept Pathol 1, Wakayama, Japan

[10] Osaka Univ, Microbial Dis Res Inst, Dept Tumor Virol, Osaka, Japan

来源：

ARCHIVES OF VIROLOGY | 1999年 / 144卷 / 01期

关键词：

D O I：

10.1007/s007050050492

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Unlike other herpesviruses, Epstein-Barr virus (EBV) has not yet been shown to infect macrophages. Six macrophage cultures were isolated from normal and affected samples. Nested polymerase chain reaction revealed the existence of the EBV genome in all these macrophages. EBV latent genes expression in all cultures were detected by mRNA in situ hybridization and immunofluorescence staining. Some cultures also expressed EBV replicative-infection proteins, while in other cultures induction of these proteins was demonstrated. These findings are the first to show expression of several latent and replicative-infection genes of EBV in macrophages, indicating that EBV proliferates in macrophages.

引用

页码：157 / 166

页数：10