Biodegradation of nicotine by a novel strain Pusillimonas

被引：19

作者：

Ma, Yun ^{[1
]}

Wen, Rongti ^{[1
]}

Qiu, Jiguo ^{[1
]}

Hong, Jun ^{[1
]}

Liu, Meng ^{[1
]}

Zhang, Dou ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Biol & Environm Engn, Res Ctr Environm Sci, Hangzhou 310032, Zhejiang, Peoples R China

来源：

RESEARCH IN MICROBIOLOGY | 2015年 / 166卷 / 02期

关键词：

Nicotine; Degradation pathway; Pusillimonas sp T2; Intermediates; BACTERIAL OXIDATION; GENE; IDENTIFICATION; DEGRADATION; PRODUCT;

D O I：

10.1016/j.resmic.2014.12.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel strain, Pusillimonas sp. T2, which is capable of degrading nicotine, was isolated and identified. This strain could completely degrade 500 mg/L nicotine within 8 h at 30 degrees C, pH 7.0. Six intermediates were detected and identified as 6-hydroxy-nicotine, 6-hydroxy-N-methylmyosmine, 6-hydroxypseudooxynicotine, 2,6-dihydroxypyridine, 6-hydroxy-3-succinoyl-pyridine and 2,5-dihydroxypyridine. Activities of 6-hydroxy-3-succinoyl-pyridine hydroxylase and 2,6-dihydroxypyridine hydroxylase were demonstrated in the cell extracts of strain T2, indicating that this strain may employ a novel variant of the pyridine and pyrrolidine pathways that is different from those of other species. This study provides the first evidence that Pusillimonas bacteria participate in nicotine degradation. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

引用

页码：67 / 71

页数：5

共 18 条

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