Identification of candidate genes involved in the sugar metabolism and accumulation during pear fruit post-harvest ripening of 'Red Clapp's Favorite' (Pyrus communis L.) by transcriptome analysis

被引：17

作者：

Wang, Long ^{[1
]}

Chen, Yun ^{[1
]}

Wang, Suke ^{[1
]}

Xue, Huabai ^{[1
]}

Su, Yanli ^{[1
]}

Yang, Jian ^{[1
]}

Li, Xiugen ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Zhengzhou Fruit Res Inst, Weilai Rd South End, Zhengzhou 450009, Henan, Peoples R China

来源：

HEREDITAS | 2017年 / 155卷

关键词：

Pear (Pyrus communis L.); Quantitative real-time PCR (qPCR); RNA-Seq; Sugar determination; EXPRESSION; BIOSYNTHESIS; MECHANISMS;

D O I：

10.1186/s41065-017-0046-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Pear (Pyrus spp.) is a popular fruit that is commercially cultivated in most temperate regions. In fruits, sugar metabolism and accumulation are important factors for fruit organoleptic quality. Post-harvest ripening is a special feature of 'Red Clapp's Favorite'. Results: In this study, transcriptome sequencing based on the Illumina platform generated 23.8 - 35.8 million unigenes of nine cDNA libraries constructed using RNAs from the 'Red Clapp's Favorite' pear variety with different treatments, in which 2629 new genes were discovered, and 2121 of them were annotated. A total of 2146 DEGs, 3650 DEGs, 1830 DEGs from each comparison were assembled. Moreover, the gene expression patterns of 8 unigenes related to sugar metabolism revealed by qPCR. The main constituents of soluble sugars were fructose and glucose after pear fruit post-harvest ripening, and five unigenes involved in sugar metabolism were discovered. Conclusions: Our study not only provides a large-scale assessment of transcriptome resources of 'Red Clapp's Favorite' but also lays the foundation for further research into genes correlated with sugar metabolism.

引用

页数：10

共 36 条

[11] Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain [J].

Li, Ping ;

Wind, Julia J. ;

Shi, Xiaoliang ;

Zhang, Honglei ;

Hanson, Johannes ;

Smeekens, Sjef C. ;

Teng, Sheng .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (08) :3436-3441

[12] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[13] Transcriptomic analysis of Arabidopsis developing stems: a close-up on cell wall genes [J].

Minic, Zoran ;

Jamet, Elisabeth ;

San-Clemente, Helene ;

Pelletier, Sandra ;

Renou, Jean-Pierre ;

Rihouey, Christophe ;

Okinyo, Denis P. O. ;

Proux, Caroline ;

Lerouge, Patrice ;

Jouanin, Lise .

BMC PLANT BIOLOGY, 2009, 9

[14] Glucose and Auxin Signaling Interaction in Controlling Arabidopsis thaliana Seedlings Root Growth and Development [J].

Mishra, Bhuwaneshwar S. ;

Singh, Manjul ;

Aggrawal, Priyanka ;

Laxmi, Ashverya .

PLOS ONE, 2009, 4 (02)

[15] Mapping and quantifying mammalian transcriptomes by RNA-Seq [J].

Mortazavi, Ali ;

Williams, Brian A. ;

McCue, Kenneth ;

Schaeffer, Lorian ;

Wold, Barbara .

NATURE METHODS, 2008, 5 (07) :621-628

[16] ACTIVITIES OF ENZYMES INVOLVED IN SUGAR METABOLISM IN PASSE-CRASSANE PEARS DURING COLD STORAGE [J].

PECH, JC ;

LATCHE, A .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1972, 23 (12) :1499-1502

[17] Sensory evaluation from a consumer perspective and its application to 'Abate Fetel' pear fruit quality [J].

Predieri, S ;

Gatti, E ;

Rapparini, F ;

Cavicchi, L ;

Colombo, R .

Proceedings of the 9th International Pear Symposium, 2005, (671) :349-353

[18] Sugar sensing and signaling in plants: Conserved and novel mechanisms [J].

Rolland, Filip ;

Baena-Gonzalez, Elena ;

Sheen, Jen .

ANNUAL REVIEW OF PLANT BIOLOGY, 2006, 57 :675-709

[19] Licorice β-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin [J].

Seki, Hikaru ;

Ohyama, Kiyoshi ;

Sawai, Satoru ;

Mizutani, Masaharu ;

Ohnishi, Toshiyuki ;

Sudo, Hiroshi ;

Akashi, Tomoyoshi ;

Aoki, Toshio ;

Saito, Kazuki ;

Muranaka, Toshiya .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (37) :14204-14209

[20]

Stebbins RL, 1978, PICKING STORING APPL

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