Establishment of a novel high-affinity IgE receptor-positive canine mast cell line with wild type c-kit receptors

被引：11
作者：
Amagai, Yosuke [1 ]
Tanaka, Akane [1 ]
Ohmori, Keitaro [1 ]
Matsuda, Hiroshi [1 ]
机构：
[1] Tokyo Univ Agr & Technol, Inst Symbiot Sci & Technol, Grad Sch, Lab Vet Mol Pathol & Therapeut,Div Anim Life Sci, Tokyo 1838509, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 03期
基金：
日本学术振兴会;
关键词：
mast cell; Fc epsilon RI; c-kit; degranulation;
D O I：
10.1016/j.bbrc.2007.12.053
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Much is known regarding participations of mast cells with innate and acquired immunity by secreting various cytokines and chemical mediators. However, details of mast cell biology still remain unclear. In this study, we successfully established a novel growth factor-independent mast cell line (MPT-1) derived from canine mast cell tumor. MPT-1 cells manifested factor-independent proliferation as floating cells containing a large amount of histamine, as well as chymase-like dog mast cell protease 3, in cytosolic granules. Particularly, MPT-1 cells expressed high-affinity IgE receptors (Fc epsilon RI) and wild-type c-kit receptors. Degranulation of MPT-1 cells was induced not only by stimulation with calcium ionophore but also by cross-linkage of the surface IgE. Given that MPT-1 is the first mast cell line with Fc epsilon RI which has no c-kit mutations, MPT-1 cells may provide great contribution for investigation of IgE-mediated activation mechanisms of mast cells, leading to development of effective treatment for allergic disorders. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：857 / 861
页数：5
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