Expressed sequence tag analysis of Eimeria-stimulated intestinal intraepithelial lymphocytes in chickens

被引：31
作者：
Min, WG
Lillehoj, HS [1 ]
Ashwell, CM
van Tassell, CP
Dalloul, RA
Matukumalli, LK
Han, JY
Lillehoj, EP
机构：
[1] USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA
[2] USDA, Growth Biol Lab, Beltsville, MD 20705 USA
[3] USDA, Bovine Funct Genom Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA
[4] Seoul Natl Univ, Sch Agr Biotecnol, Seoul 151742, South Korea
[5] George Mason Univ, Fairfax, VA 22030 USA
[6] Univ Maryland, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA
[7] Sunchon Natl Univ, Dept Anim Sci & Technol, Sunchon 540742, Chonnam, South Korea
来源：
MOLECULAR BIOTECHNOLOGY | 2005年 / 30卷 / 02期
关键词：
chickens; intestinal lymphocytes; expressed sequence tag (EST); expression cDNA library; Eimeria;
D O I：
10.1385/MB:30:2:143
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Intraepithelial lymphocytes (IELs) play a critical role in protective immune response to intestinal pathogens such as Eimeria, the etiologic agent of avian coccidiosis. A list of genes expressed by intestinal IELs of Eimeria-infected chickens was compiled using the expressed sequence tag (EST) strategy. The 14,409 ESTs consisted of 1851 clusters and 7595 singletons, which revealed 9446 unique genes in the data,set. Comparison of the sequence data with chicken DNA sequences in GenBank identified 125 novel clones. This EST library will provide a valuable resource for profiling global gene expression in normal and pathogen-infected chickens and identifying additional unique immune-related genes.
引用
收藏
页码：143 / 149
页数：7
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