Immunological and pathological outcomes of SARS-CoV-2 challenge following formalin-inactivated vaccine in ferrets and rhesus macaques

被引：19

作者：

Bewley, Kevin R. ^{[1
]}

Gooch, Karen ^{[1
]}

Thomas, Kelly M. ^{[1
]}

Longet, Stephanie ^{[1
]}

Wiblin, Nathan ^{[1
]}

Hunter, Laura ^{[1
]}

Chan, Kin ^{[1
]}

Brown, Phillip ^{[1
]}

Russell, Rebecca A. ^{[2
]}

Ho, Catherine ^{[1
]}

Slack, Gillian ^{[1
]}

Humphries, Holly E. ^{[1
]}

Alden, Leonie ^{[1
]}

Allen, Lauren ^{[1
]}

Aram, Marilyn ^{[1
]}

Baker, Natalie ^{[1
]}

Brunt, Emily ^{[1
]}

Cobb, Rebecca ^{[1
]}

Fotheringham, Susan ^{[1
]}

Harris, Debbie ^{[1
]}

Kennard, Chelsea ^{[1
]}

Leung, Stephanie ^{[1
]}

Ryan, Kathryn ^{[1
]}

Tolley, Howard ^{[1
]}

Wand, Nadina ^{[1
]}

White, Andrew ^{[1
]}

Sibley, Laura ^{[1
]}

Sarfas, Charlotte ^{[1
]}

Pearson, Geoff ^{[1
]}

Rayner, Emma ^{[1
]}

Xue, Xiaochao ^{[2
]}

Lambe, Teresa ^{[3
]}

Charlton, Sue ^{[1
]}

Gilbert, Sarah ^{[3
]}

Sattentau, Quentin J. ^{[2
]}

Gleeson, Fergus ^{[4
]}

Hall, Yper ^{[1
]}

Funnell, Simon ^{[1
,5
]}

Sharpe, Sally ^{[1
]}

Salguero, Francisco J. ^{[1
]}

Gorringe, Andrew ^{[1
]}

Carroll, Miles ^{[1
,6
]}

机构：

[1] Publ Hlth England, Salisbury SP4 0JG, Wilts, England

[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[3] Univ Oxford, Jenner Inst, Nuffield Dept Med, Oxford OX3 7DQ, England

[4] Oxford Univ Hosp NHS Fdn Trust, Oxford Dept Radiol & Nucl Med, Oxford OX3 7LE, England

[5] Quadram Inst Biosci, Norwich Res Pk, Norwich, Norfolk, England

[6] Univ Oxford, Pandem Preparedness Ctr, Nuffield Dept Med, Oxford OX3 7LG, England

来源：

SCIENCE ADVANCES | 2021年 / 7卷 / 37期

关键词：

ACUTE RESPIRATORY SYNDROME; CORONAVIRUS VACCINE; VIRUS-INFECTION; BALB/C MICE; T-CELLS; DISEASE; GLYCOPROTEIN; IMMUNIZATION; ENHANCEMENT; ANTIBODIES;

D O I：

10.1126/sciadv.abg7996

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

There is an urgent requirement for safe and effective vaccines to prevent COVID-19. A concern for the development of new viral vaccines is the potential to induce vaccine-enhanced disease (VED). This was reported in several preclinical studies with both SARS-CoV-1 and MERS vaccines but has not been reported with SARS-CoV-2 vaccines. We have used ferrets and rhesus macaques challenged with SARS-CoV-2 to assess the potential for VED in animals vaccinated with formaldehyde-inactivated SARS-CoV-2 (FIV) formulated with Alhydrogel, compared to a negative control vaccine. We showed no evidence of enhanced disease in ferrets or rhesus macaques given FIV except for mild transient enhanced disease seen 7 days after infection in ferrets. This increased lung pathology was observed at day 7 but was resolved by day 15. We also demonstrate that formaldehyde treatment of SARS-CoV-2 reduces exposure of the spike receptor binding domain providing a mechanistic explanation for suboptimal immunity.

引用

页数：17

共 64 条

[1] Brief History and Characterization of Enhanced Respiratory Syncytial Virus Disease [J].

Acosta, Patricio L. ;

Caballero, Mauricio T. ;

Polack, Fernando P. .

CLINICAL AND VACCINE IMMUNOLOGY, 2016, 23 (03) :189-195

[2] Role of PD-1 during effector CD8 T cell differentiation [J].

Ahn, Eunseon ;

Araki, Koichi ;

Hashimoto, Masao ;

Li, Weiyan ;

Riley, James L. ;

Cheung, Jeanne ;

Sharpe, Arlene H. ;

Freeman, Gordon J. ;

Irving, Bryan A. ;

Ahmed, Rafi .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (18) :4749-4754

[3]

Amanat Fatima, 2020, Curr Protoc Microbiol, V58, pe108, DOI 10.1002/cpmc.108

[4] A perspective on potential antibody-dependent enhancement of SARS-CoV-2 [J].

Arvin, Ann M. ;

Fink, Katja ;

Schmid, Michael A. ;

Cathcart, Andrea ;

Spreafico, Roberto ;

Havenar-Daughton, Colin ;

Lanzavecchia, Antonio ;

Corti, Davide ;

Virgin, Herbert W. .

NATURE, 2020, 584 (7821) :353-363

[5] Distinct Early Serological Signatures Track with SARS-CoV-2 Survival [J].

Atyeo, Caroline ;

Fischinger, Stephanie ;

Zohar, Tomer ;

Slein, Matthew D. ;

Burke, John ;

Loos, Carolin ;

McCulloch, Denise J. ;

Newman, Kira L. ;

Wolf, Caitlin ;

Yu, Jingyou ;

Shuey, Kiel ;

Feldman, Jared ;

Hauser, Blake Marie ;

Caradonna, Tim ;

Schmidt, Aaron G. ;

Suscovich, Todd J. ;

Linde, Caitlyn ;

Cai, Yongfei ;

Barouch, Dan ;

Ryan, Edward T. ;

Charles, Richelle C. ;

Lauffenburger, Douglas ;

Chu, Helen ;

Alter, Galit .

IMMUNITY, 2020, 53 (03) :524-+

[6] Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine [J].

Baden, Lindsey R. ;

El Sahly, Hana M. ;

Essink, Brandon ;

Kotloff, Karen ;

Frey, Sharon ;

Novak, Rick ;

Diemert, David ;

Spector, Stephen A. ;

Rouphael, Nadine ;

Creech, C. Buddy ;

McGettigan, John ;

Khetan, Shishir ;

Segall, Nathan ;

Solis, Joel ;

Brosz, Adam ;

Fierro, Carlos ;

Schwartz, Howard ;

Neuzil, Kathleen ;

Corey, Larry ;

Gilbert, Peter ;

Janes, Holly ;

Follmann, Dean ;

Marovich, Mary ;

Mascola, John ;

Polakowski, Laura ;

Ledgerwood, Julie ;

Graham, Barney S. ;

Bennett, Hamilton ;

Pajon, Rolando ;

Knightly, Conor ;

Leav, Brett ;

Deng, Weiping ;

Zhou, Honghong ;

Han, Shu ;

Ivarsson, Melanie ;

Miller, Jacqueline ;

Zaks, Tal .

NEW ENGLAND JOURNAL OF MEDICINE, 2021, 384 (05) :403-416

[7] Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays [J].

Bewley, Kevin R. ;

Coombes, Naomi S. ;

Gagnon, Luc ;

McInroy, Lorna ;

Baker, Natalie ;

Shaik, Imam ;

St-Jean, Julien R. ;

St-Amant, Natalie ;

Buttigieg, Karen R. ;

Humphries, Holly E. ;

Godwin, Kerry J. ;

Brunt, Emily ;

Allen, Lauren ;

Leung, Stephanie ;

Brown, Phillip J. ;

Penn, Elizabeth J. ;

Thomas, Kelly ;

Kulnis, Greg ;

Hallis, Bassam ;

Carroll, Miles ;

Funnell, Simon ;

Charlton, Sue .

NATURE PROTOCOLS, 2021, 16 (06) :3114-+

[8] A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response [J].

Bolles, Meagan ;

Deming, Damon ;

Long, Kristin ;

Agnihothram, Sudhakar ;

Whitmore, Alan ;

Ferris, Martin ;

Funkhouser, William ;

Gralinski, Lisa ;

Totura, Allison ;

Heise, Mark ;

Baric, Ralph S. .

JOURNAL OF VIROLOGY, 2011, 85 (23) :12201-12215

[9] Guidelines for collection, analysis and presentation of vaccine safety data in pre- and post-licensure clinical studies [J].

Bonhoeffer, Jan ;

Bentsi-Enchill, Adwoa ;

Chen, Robert T. ;

Fisher, Margaret C. ;

Gold, Michael S. ;

Hartman, Katharina ;

Heininger, Ulrich ;

Hoet, Bernard ;

Jefferson, Thomas ;

Khuri-Bulos, Najwa ;

Kohl, Katrin S. ;

Marcy, S. Michael ;

Nalin, David ;

Pless, Robert ;

Sanabria-Rojas, Hernan ;

Sleeman, Karen ;

Wise, Robert .

VACCINE, 2009, 27 (16) :2282-2288

[10] Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia [J].

Caly, Leon ;

Druce, Julian ;

Roberts, Jason ;

Bond, Katherine ;

Tran, Thomas ;

Kostecki, Renata ;

Yoga, Yano ;

Naughton, William ;

Taiaroa, George ;

Seemann, Torsten ;

Schultz, Mark B. ;

Howden, Benjamin P. ;

Korman, Tony M. ;

Lewin, Sharon R. ;

Williamson, Deborah A. ;

Catton, Mike G. .

MEDICAL JOURNAL OF AUSTRALIA, 2020, 212 (10) :459-462

← 1 2 3 4 5 6 7 →