Immunological and pathological outcomes of SARS-CoV-2 challenge following formalin-inactivated vaccine in ferrets and rhesus macaques

被引:18
作者
Bewley, Kevin R. [1 ]
Gooch, Karen [1 ]
Thomas, Kelly M. [1 ]
Longet, Stephanie [1 ]
Wiblin, Nathan [1 ]
Hunter, Laura [1 ]
Chan, Kin [1 ]
Brown, Phillip [1 ]
Russell, Rebecca A. [2 ]
Ho, Catherine [1 ]
Slack, Gillian [1 ]
Humphries, Holly E. [1 ]
Alden, Leonie [1 ]
Allen, Lauren [1 ]
Aram, Marilyn [1 ]
Baker, Natalie [1 ]
Brunt, Emily [1 ]
Cobb, Rebecca [1 ]
Fotheringham, Susan [1 ]
Harris, Debbie [1 ]
Kennard, Chelsea [1 ]
Leung, Stephanie [1 ]
Ryan, Kathryn [1 ]
Tolley, Howard [1 ]
Wand, Nadina [1 ]
White, Andrew [1 ]
Sibley, Laura [1 ]
Sarfas, Charlotte [1 ]
Pearson, Geoff [1 ]
Rayner, Emma [1 ]
Xue, Xiaochao [2 ]
Lambe, Teresa [3 ]
Charlton, Sue [1 ]
Gilbert, Sarah [3 ]
Sattentau, Quentin J. [2 ]
Gleeson, Fergus [4 ]
Hall, Yper [1 ]
Funnell, Simon [1 ,5 ]
Sharpe, Sally [1 ]
Salguero, Francisco J. [1 ]
Gorringe, Andrew [1 ]
Carroll, Miles [1 ,6 ]
机构
[1] Publ Hlth England, Salisbury SP4 0JG, Wilts, England
[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
[3] Univ Oxford, Jenner Inst, Nuffield Dept Med, Oxford OX3 7DQ, England
[4] Oxford Univ Hosp NHS Fdn Trust, Oxford Dept Radiol & Nucl Med, Oxford OX3 7LE, England
[5] Quadram Inst Biosci, Norwich Res Pk, Norwich, Norfolk, England
[6] Univ Oxford, Pandem Preparedness Ctr, Nuffield Dept Med, Oxford OX3 7LG, England
关键词
ACUTE RESPIRATORY SYNDROME; CORONAVIRUS VACCINE; VIRUS-INFECTION; BALB/C MICE; T-CELLS; DISEASE; GLYCOPROTEIN; IMMUNIZATION; ENHANCEMENT; ANTIBODIES;
D O I
10.1126/sciadv.abg7996
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
There is an urgent requirement for safe and effective vaccines to prevent COVID-19. A concern for the development of new viral vaccines is the potential to induce vaccine-enhanced disease (VED). This was reported in several preclinical studies with both SARS-CoV-1 and MERS vaccines but has not been reported with SARS-CoV-2 vaccines. We have used ferrets and rhesus macaques challenged with SARS-CoV-2 to assess the potential for VED in animals vaccinated with formaldehyde-inactivated SARS-CoV-2 (FIV) formulated with Alhydrogel, compared to a negative control vaccine. We showed no evidence of enhanced disease in ferrets or rhesus macaques given FIV except for mild transient enhanced disease seen 7 days after infection in ferrets. This increased lung pathology was observed at day 7 but was resolved by day 15. We also demonstrate that formaldehyde treatment of SARS-CoV-2 reduces exposure of the spike receptor binding domain providing a mechanistic explanation for suboptimal immunity.
引用
收藏
页数:17
相关论文
共 64 条
[1]   Brief History and Characterization of Enhanced Respiratory Syncytial Virus Disease [J].
Acosta, Patricio L. ;
Caballero, Mauricio T. ;
Polack, Fernando P. .
CLINICAL AND VACCINE IMMUNOLOGY, 2016, 23 (03) :189-195
[2]   Role of PD-1 during effector CD8 T cell differentiation [J].
Ahn, Eunseon ;
Araki, Koichi ;
Hashimoto, Masao ;
Li, Weiyan ;
Riley, James L. ;
Cheung, Jeanne ;
Sharpe, Arlene H. ;
Freeman, Gordon J. ;
Irving, Bryan A. ;
Ahmed, Rafi .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (18) :4749-4754
[3]  
Amanat Fatima, 2020, Curr Protoc Microbiol, V58, pe108, DOI 10.1002/cpmc.108
[4]   A perspective on potential antibody-dependent enhancement of SARS-CoV-2 [J].
Arvin, Ann M. ;
Fink, Katja ;
Schmid, Michael A. ;
Cathcart, Andrea ;
Spreafico, Roberto ;
Havenar-Daughton, Colin ;
Lanzavecchia, Antonio ;
Corti, Davide ;
Virgin, Herbert W. .
NATURE, 2020, 584 (7821) :353-363
[5]   Distinct Early Serological Signatures Track with SARS-CoV-2 Survival [J].
Atyeo, Caroline ;
Fischinger, Stephanie ;
Zohar, Tomer ;
Slein, Matthew D. ;
Burke, John ;
Loos, Carolin ;
McCulloch, Denise J. ;
Newman, Kira L. ;
Wolf, Caitlin ;
Yu, Jingyou ;
Shuey, Kiel ;
Feldman, Jared ;
Hauser, Blake Marie ;
Caradonna, Tim ;
Schmidt, Aaron G. ;
Suscovich, Todd J. ;
Linde, Caitlyn ;
Cai, Yongfei ;
Barouch, Dan ;
Ryan, Edward T. ;
Charles, Richelle C. ;
Lauffenburger, Douglas ;
Chu, Helen ;
Alter, Galit .
IMMUNITY, 2020, 53 (03) :524-+
[6]   Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine [J].
Baden, Lindsey R. ;
El Sahly, Hana M. ;
Essink, Brandon ;
Kotloff, Karen ;
Frey, Sharon ;
Novak, Rick ;
Diemert, David ;
Spector, Stephen A. ;
Rouphael, Nadine ;
Creech, C. Buddy ;
McGettigan, John ;
Khetan, Shishir ;
Segall, Nathan ;
Solis, Joel ;
Brosz, Adam ;
Fierro, Carlos ;
Schwartz, Howard ;
Neuzil, Kathleen ;
Corey, Larry ;
Gilbert, Peter ;
Janes, Holly ;
Follmann, Dean ;
Marovich, Mary ;
Mascola, John ;
Polakowski, Laura ;
Ledgerwood, Julie ;
Graham, Barney S. ;
Bennett, Hamilton ;
Pajon, Rolando ;
Knightly, Conor ;
Leav, Brett ;
Deng, Weiping ;
Zhou, Honghong ;
Han, Shu ;
Ivarsson, Melanie ;
Miller, Jacqueline ;
Zaks, Tal .
NEW ENGLAND JOURNAL OF MEDICINE, 2021, 384 (05) :403-416
[7]   Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays [J].
Bewley, Kevin R. ;
Coombes, Naomi S. ;
Gagnon, Luc ;
McInroy, Lorna ;
Baker, Natalie ;
Shaik, Imam ;
St-Jean, Julien R. ;
St-Amant, Natalie ;
Buttigieg, Karen R. ;
Humphries, Holly E. ;
Godwin, Kerry J. ;
Brunt, Emily ;
Allen, Lauren ;
Leung, Stephanie ;
Brown, Phillip J. ;
Penn, Elizabeth J. ;
Thomas, Kelly ;
Kulnis, Greg ;
Hallis, Bassam ;
Carroll, Miles ;
Funnell, Simon ;
Charlton, Sue .
NATURE PROTOCOLS, 2021, 16 (06) :3114-+
[8]   A Double-Inactivated Severe Acute Respiratory Syndrome Coronavirus Vaccine Provides Incomplete Protection in Mice and Induces Increased Eosinophilic Proinflammatory Pulmonary Response [J].
Bolles, Meagan ;
Deming, Damon ;
Long, Kristin ;
Agnihothram, Sudhakar ;
Whitmore, Alan ;
Ferris, Martin ;
Funkhouser, William ;
Gralinski, Lisa ;
Totura, Allison ;
Heise, Mark ;
Baric, Ralph S. .
JOURNAL OF VIROLOGY, 2011, 85 (23) :12201-12215
[9]   Guidelines for collection, analysis and presentation of vaccine safety data in pre- and post-licensure clinical studies [J].
Bonhoeffer, Jan ;
Bentsi-Enchill, Adwoa ;
Chen, Robert T. ;
Fisher, Margaret C. ;
Gold, Michael S. ;
Hartman, Katharina ;
Heininger, Ulrich ;
Hoet, Bernard ;
Jefferson, Thomas ;
Khuri-Bulos, Najwa ;
Kohl, Katrin S. ;
Marcy, S. Michael ;
Nalin, David ;
Pless, Robert ;
Sanabria-Rojas, Hernan ;
Sleeman, Karen ;
Wise, Robert .
VACCINE, 2009, 27 (16) :2282-2288
[10]   Isolation and rapid sharing of the 2019 novel coronavirus (SAR-CoV-2) from the first patient diagnosed with COVID-19 in Australia [J].
Caly, Leon ;
Druce, Julian ;
Roberts, Jason ;
Bond, Katherine ;
Tran, Thomas ;
Kostecki, Renata ;
Yoga, Yano ;
Naughton, William ;
Taiaroa, George ;
Seemann, Torsten ;
Schultz, Mark B. ;
Howden, Benjamin P. ;
Korman, Tony M. ;
Lewin, Sharon R. ;
Williamson, Deborah A. ;
Catton, Mike G. .
MEDICAL JOURNAL OF AUSTRALIA, 2020, 212 (10) :459-462