Development and validation of a new loop-mediated isothermal amplification for detection of pathogenic Leptospira species in clinical materials

被引：14

作者：

Hsu, Yun-Hsiu ^{[1
]}

Chou, Shih-Jen ^{[1
]}

Chang, Chih-Cheng ^{[1
]}

Pan, Ming-Jeng ^{[2
]}

Yang, Wei-Cheng ^{[1
]}

Lin, Chuen-Fu ^{[1
]}

Chan, Kun-Wei ^{[1
]}

机构：

[1] Natl Chiayi Univ, Coll Vet Med, Chiayi, Taiwan

[2] Cent Taiwan Univ Sci & Technol, Coll Hlth Sci, Taichung, Taiwan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2017年 / 141卷

关键词：

Leptospira; Loop-mediated isothermal amplification; (LAMP); DNA detection; POLYMERASE-CHAIN-REACTION; REACTION ASSAY; PCR; DIAGNOSIS; THAILAND; PRIMERS;

D O I：

10.1016/j.mimet.2017.07.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Leptospirosis, a global zoonotic disease, is often neglected but has a significant impact on human health. Here we reported a new loop mediated isothermal amplification (LAMP) assay targeting lipL32 gene of pathogenic Leptospira spp. Polymerase chain reaction (PCR), nested-PCR and real-time PCR assays were included in this study for the comparison of analytic and diagnostic sensitivity and specificity. LipL32 LAMP we designed enables detection of 10 copies of L. interrogans and has a higher diagnostic sensitivity (91.67%) and specificity (100%) than other PCR-based methods. The high sensitivity, specificity and flexible reaction conditions of the lipL32 LAMP assay makes it feasible for resource -limited countries and on-site application.

引用

收藏

页码：55 / 59

页数：5

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