Liver sinusoidal endothelial and biliary cell repopulation following irradiation and partial hepatectomy

被引:12
作者
Krause, Petra [1 ]
Rave-Fraenk, Margret [2 ]
Wolff, Hendrik Andreas [2 ]
Becker, Heinz [1 ]
Christiansen, Hans [2 ]
Koenig, Sarah [1 ]
机构
[1] Univ Med Ctr Goettingen, Dept Gen Surg, D-37099 Gottingen, Germany
[2] Univ Med Ctr Goettingen, Dept Radiotherapy, D-37099 Gottingen, Germany
关键词
Cell transplants; Dipeptidyl peptidase IV protein; Endothelial cells; Liver cell transplantation; Liver irradiation; Liver repopulation; HEPATOCYTE TRANSPLANTATION; PREPARATIVE REGIMEN; REPERFUSION INJURY; RADIATION-THERAPY; MITOMYCIN-C; RATS; REGENERATION; RADIOTHERAPY; ENGRAFTMENT; METASTASES;
D O I
10.3748/wjg.v16.i31.3928
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Aim: To investigate whether irradiation (IR) and partial hepatectomy (PH) may prepare the host liver for nonparenchymal cell (NPC) transplantation. METHODS: Livers of dipeptidyl peptidase IV (DPP IV)-deficient rats were pre-conditioned with external beam IR (25 Gy) delivered to two-thirds of the right liver lobules followed by a one-third PH of the untreated lobule. DPPIV-positive liver cells (NPC preparations enriched for liver sinusoidal endothelial cells (LSECs) and hepatocytes) were transplanted via the spleen into the recipient livers. The extent and quality of donor cell engraftment and growth was studied over a long-term interval of 16 wk after transplantation. RESULTS: Host liver staining demonstrated 3 different repopulation types. Well defined clusters of donor-derived hepatocytes with canalicular expression of DPPIV were detectable either adjacent to or in between large areas of donor cells (covering up to 90% of the section plane) co-expressing the endothelial marker platelet endothelial cell adhesion molecule. The third type consisted of formations of DPPIV-positive duct-like structures which colocalized with biliary epithelial CD49f. CONCLUSION: Liver IR and PH as a preconditioning stimulus enables multiple cell liver repopulation by donor hepatocytes, LSECs, and bile duct cells. (C) 2010 Baishideng. All rights reserved.
引用
收藏
页码:3928 / 3935
页数:8
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