DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death

被引：61

作者：

Bonassi, Stefano ^{[1
,2
]}

Ceppi, Marcello ^{[3
]}

Moller, Peter ^{[4
]}

Azqueta, Amaya ^{[5
,6
]}

Milic, Mirta ^{[7
]}

Monica, Neri ^{[1
]}

Brunborg, Gunnar ^{[8
]}

Godschalk, Roger ^{[9
]}

Koppen, Gudrun ^{[10
]}

Langie, Sabine A. S. ^{[9
]}

Teixeira, Joao Paulo ^{[11
,12
,13
]}

Bruzzone, Marco

Da Silva, Juliana ^{[14
]}

Benedetti, Danieli ^{[14
]}

Cavallo, Delia ^{[15
]}

Ursini, Cinzia Lucia ^{[15
]}

Giovannelli, Lisa ^{[16
]}

Moretti, Silvia ^{[17
]}

Riso, Patrizia ^{[18
]}

Del Bo', Cristian ^{[18
]}

Russo, Patrizia ^{[1
,2
]}

Dobrzynska, Malgorzata ^{[19
]}

Goroshinskaya, Irina A. ^{[20
]}

Surikova, Ekaterina I. ^{[20
]}

Staruchova, Marta ^{[21
]}

Barancokova, Magdalena ^{[21
]}

Volkovova, Katarina ^{[21
]}

Kazimirova, Alena ^{[21
]}

Smolkova, Bozena ^{[22
]}

Laffon, Blanca ^{[23
,24
]}

Valdiglesias, Vanessa ^{[24
,25
]}

Pastor, Susana ^{[26
]}

Marcos, Ricard ^{[26
,27
]}

Hernandez, Alba ^{[26
,27
]}

Gajski, Goran

Spremo-Potparevic, Biljana ^{[28
]}

Zivkovic, Lada ^{[28
]}

Boutet-Robinet, Elisa ^{[29
]}

Perdry, Herve ^{[30
]}

Lebailly, Pierre ^{[31
]}

Perez, Carlos L. ^{[32
]}

Basaran, Nursen

Nemeth, Zsuzsanna ^{[33
,34
]}

Safar, Anna ^{[34
]}

Dusinska, Maria ^{[35
]}

Collins, Andrew ^{[36
]}

Anderson, Diana ^{[37
]}

Andrade, Vanessa ^{[38
]}

Pereira, Cristiana Costa ^{[39
]}

Costa, Solange ^{[39
]}

机构：

[1] IRCCS San Raffaele Roma, Unit Clin & Mol Epidemiol, Rome, Italy

[2] San Raffaele Univ, Dept Human Sci & Qual Life Promot, Unit Clin & Mol Epidemiol, IRCCS San Raffaele Roma, Via Val Cannuta, I-00166 Rome, Italy

[3] San Martino Policlin Hosp, Clin Epidemiol Unit, Genoa, Italy

[4] Univ Copenhagen, Environm Hlth Sect, Dept Publ Hlth, Oster Farimagsgade 5A, DK-1014 Copenhagen, Denmark

[5] Univ Navarra, Dept Pharmacol & Toxicol, C Irunlarrea 1, Pamplona 31008, Spain

[6] Navarra Inst Hlth Res, IdiSNA, C Irunlarrea 3, Pamplona 31008, Spain

[7] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Ksaverska Cesta 2, Zagreb 10000, Croatia

[8] Norwegian Inst Publ Hlth NIPH, Sect Mol Toxicol, Dept Environm Hlth, Lovisenberggt 6, N-0456 Oslo, Norway

[9] Maastricht Univ, Sch Nutr & Translat Res Metab, Dept Pharmacol & Toxicol, Univ 50, NL-6200 MD Maastricht, Netherlands

[10] Flemish Inst Technol Res, Environm Risk & Hlth Unit VITO BIOMo, Mol, Belgium

[11] Natl Inst Hlth, Dept Environm Hlth, Rua Alexandre Herculano,321, P-4000055 Porto, Portugal

[12] Inst Nacl Saude Doutor Ricardo Jorge, Dept Environm Hlth, Rua Alexandre Herculano 321, P-4000055 Porto, Portugal

[13] Univ Porto, EPIUnit Inst Saude Publ, Rua Taipas, P-4050600 Porto, Portugal

[14] Lutheran Univ Brazil ULBRA, Salle Univ UNILASALLE, Lab Genet Toxicol, Canoas, RS, Brazil

[15] Italian Workers Compensat Author INAIL, Epidemiol & Hyg DiMEILA, Department Occupat & Environm Med, Via Fontana Candida 1, I-00078 Rome, Italy

[16] Univ Florence, Dept NEUROFARBA, Viale G Pieraccini 6, I-50139 Florence, Italy

[17] Univ Florence, Palagi Hosp, Div Dermatol, Dept Hlth Sci, Florence, Italy

[18] Univ Milan, Dept Food Environm & Nutrit Sci DeFENS, Via Celoria 2, I-20133 Milan, Italy

[19] Natl Inst Publ Hlth NIH Natl Res Inst, Dept Radiat Hyg & Radiobiol, 24 Chocimska St, PL-00791 Warsaw, Poland

[20] Natl Med Res Ctr Oncol, Lab Study, Pathogenesis Malignant Tumors, 14 Iine 63, Rostov Na Donu 344037, Russia

[21] Slovak Med Univ, Fac Med, Inst Biol, Limbova 12, Bratislava 83303, Slovakia

[22] Slovak Acad Sci, Res Inst, Biomed Res Ctr, Dubravska Cesta 9, Bratislava, Slovakia

[23] Univ A Coruna, Fac Ciencias Educac, Dept Psicol, Ctr Invest Cient Avanzadas CICA, Campus Elvina S-N, Coruna 15071, Spain

[24] Inst Invest Biomed Coruna INIBIC, AE CICA INIBIC, Coruna, Spain

[25] Univ A Coruna, Fac Ciencias, Dept Biol, Ctr Invest Cient Avanzadas CICA,Grp DICOMOSA, Campus Zapateira S-N, Coruna 15071, Spain

[26] Univ Autonoma Barcelona, Fac Biosci, Dept Genet & Microbiol, Barcelona 08193, Spain

[27] Carlos III Inst Hlth, Consortium Biomed Res Epidemiol & Publ Hlth CIB, Madrid 28029, Spain

[28] Univ Belgrade, Ctr Biol Res, Fac Pharm, VojvodeStepe 450, Belgrade, Serbia

[29] Univ Toulouse, Toxalim Res Ctr Food Toxicol, INRAE, ENVT, Toulouse, France

[30] Univ Paris Saclay, CESP, Villejuif, France

[31] Univ Caen, INSERM, Normandie Ctr Francois Baclesse, ANTICIPE Unit, Ave Gen Harris, F-14076 Caen 05, France

[32] Univ Ciencias Med Habana, Inst Ciencias Basicas Preclin Vic Giron, Dept Biochem, 146 St & 31 Ave, Havana, Cuba

[33] Hacettepe Univ, Dept Pharmaceut Toxicol, Fac Pharm, Ankara, Turkey

[34] Natl Publ Hlth Ctr, Dept Non Ionizing Radiat, Anna St 5, H-1221 Budapest, Hungary

[35] Hlth Effects Lab, NILU, Kjeller, Norway

[36] Univ Oslo, Inst Basic Med Sci, Dept Nutr, Sognsvannsveien 9, N-0372 Oslo, Norway

[37] Univ Bradford, Bradford, W Yorkshire, England

[38] Univ Southern Santa Catarina, Santa Catarina, Brazil

[39] Natl Inst Hlth, Lisbon, Portugal

[40] Norwegian Inst Publ Hlth, Oslo, Norway

[41] Nova Univ, Inst Politecn Lisboa, Lisbon, Portugal

[42] Univ Perugia, Perugia, Italy

[43] Mem Sloan Kettering Canc Ctr, 1275 York Ave, New York, NY 10021 USA

[44] Univ Nacl Autonoma Mexico, Mexico City, DF, Mexico

[45] Univ Toulouse, ISA Lille, Toulouse, France

[46] Inst Nucl Chem & Technol, Warsaw, Poland

[47] Univ Vienna, Vienna, Austria

[48] Univ Oslo, Oslo, Norway

[49] Nat Inst Biol, Ljubljana, Slovenia

[50] Univ Wurzburg, Wurzburg, Germany

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

CHROMOSOMAL-ABERRATIONS; HUMAN CANCER; LYMPHOCYTES PREDICTS; FREQUENCY; VALIDATION; BIOMARKERS; HALLMARKS; ADDUCTS; REPAIR; COHORT;

D O I：

10.1038/s41598-021-95976-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.

引用

页数：11

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