Characterization of phenolic extracts from Brava extra virgin olive oils and their cytotoxic effects on MCF-7 breast cancer cells

被引：34

作者：

Reboredo-Rodriguez, Patricia ^{[1
,2
]}

Gonzalez-Barreiro, Carmen ^{[2
]}

Cancho-Grande, Beatriz ^{[2
]}

Forbes-Hernandez, Tamara Y. ^{[1
]}

Gasparrini, Massimiliano ^{[1
]}

Afrin, Sadia ^{[1
]}

Cianciosi, Danila ^{[1
]}

Carrasco-Pancorbo, Alegria ^{[3
]}

Simal-Gandara, Jesus ^{[2
]}

Giampieri, Francesca ^{[1
]}

Battino, Maurizio ^{[1
]}

机构：

[1] Univ Politecn Marche, Dipartimento Sci Clin Specialist & Odontostomatol, Fac Med, Sez Biochim, Ancona, Italy

[2] Univ Vigo, Nutr & Bromatol Grp, Dept Analyt & Food Chem, Fac Sci, Ourense Campus, E-32004 Orense, Spain

[3] Univ Granada, Dept Analyt Chem, Fac Sci, Ave Fuentenueva S-N, E-18071 Granada, Spain

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2018年 / 119卷

关键词：

Olive oil; Phenolic compounds; Cytotoxicity; Intracellular reactive oxygen species; Apoptosis; Breast cancer; MONOUNSATURATED FATTY-ACIDS; IN-VITRO; MITOCHONDRIAL-FUNCTION; ANTIOXIDANT PROPERTIES; STRAWBERRY EXTRACT; MEDITERRANEAN DIET; OXIDATIVE STRESS; APOPTOSIS; OLEUROPEIN; ROS;

D O I：

10.1016/j.fct.2018.05.026

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The aim of the present work was to evaluate the phenolic profile of the 'Brava' extra virgin olive oil and assess its potential as a "natural adjuvant" in combination with chemotherapy treatment. The total phenol content of the phenolic extracts was 764 mg gallic acid equivalents/kg and the total antioxidant capacity was 2309, 1881 and 2088 mu M trolox equivalents/kg determined by Diphenyl-l-picrylhydrazyl free radical method, Ferric Reducing Antioxidant Power and Trolox Equivalent Antioxidant Capacity assay, respectively. Secoiridoids comprised 83% of the total phenolic compounds. The main secoiridoid from oleuropein was the main isomer of oleuropein aglycone (74 mg/kg). The main secoiridoid from ligstroside was the main isomer of ligstroside aglycone (214 mg/kg). These phenolic extracts showed a significant decrease in cell viability on MCF-7 breast cancer cells in a dose and time dependent manner. 48 h-treatments with different concentrations of the extracts induced intracellular ROS generation and cell death.

引用

页码：73 / 85

页数：13

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