Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development

被引：98

作者：

Boldbaatar, Damdinsuren ^{[1
]}

Umemiya-Shirafuji, Rika ^{[1
]}

Liao, Min ^{[1
]}

Tanaka, Tetsuya ^{[1
]}

Xuan, Xuenan ^{[2
]}

Fujisaki, Kozo ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Agr, Dept Frontier Vet Sci, Kagoshima 8900065, Japan

[2] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2010年 / 56卷 / 11期

基金：

日本学术振兴会;

关键词：

Tick; Vitellogenin; mRNA expression; Native protein expression; RNA interference; ORNITHODOROS-PARKERI ACARI; MESSENGER-RNA EXPRESSION; AMERICAN DOG TICK; DERMACENTOR-VARIABILIS; FAT-BODY; MOLECULAR CHARACTERIZATION; PERIPLANETA-AMERICANA; VONWILLEBRAND-FACTOR; NUCLEOTIDE-SEQUENCE; AMBLYOMMA-HEBRAEUM;

D O I：

10.1016/j.jinsphys.2010.05.019

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Ovarian development and egg maturation are crucial processes for the success of reproduction in ticks. Three full-length cDNAs encoding the precursor of major yolk protein, vitellogenin, were obtained from cDNA libraries of the Haemaphysalis longicornis tick and designated as HIVg-1, HIVg-2 and HIVg-3. The HIVg mRNAs were found in fed females with major expression sites in the midgut, fat body and ovary. Native PAGE and Western blot demonstrated that HIVgs in the hemolymph, fat body and ovary of fed females consisted of four major polypeptides. RNAi results showed that HIVg dsRNA-injected ticks obtained lower body weight, egg weight and showed higher mortality of engorged females after blood sucking than control groups. Our results indicate that all HIVgs are essential for egg development and oviposition. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1587 / 1598

页数：12

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