Urinary excretion of creatol, an in vivo biomarker of hydroxyl radical, in patients with chronic renal failure

被引:15
作者
Ienaga, Kazuharu
Nakamura, Ko
Fujisawa, Tomoe
Fukunaga, Yasutsugu
Nihei, Hiroshi
Narita, Mitsuharu
Tomino, Yasuhiko
Sanaka, Tsutomu
Aoyagi, Kazumasa
Nakano, Koji
Koide, Hikaru
机构
[1] Koto Hosp, Dept Med, Koto Ku, Tokyo 1360072, Japan
[2] Yamasiro Hosp, Kyoto, Japan
[3] Tsukuba Coll Technol, Ibaraki, Japan
[4] Second Hosp, Tokyo Womens Med Coll, Tokyo, Japan
[5] Juntendo Univ, Sch Med, Tokyo 113, Japan
[6] Mito Cent Hosp, Ibaraki, Japan
[7] Tokyo Womens Med Coll, Tokyo 162, Japan
[8] Nippon Zoki Pharmaceut Co Ltd, Inst Bioact Sci, Hyogo, Japan
关键词
chronic renal failure; creatinine; creatol; hydroxyl radical adduct; methylguanidine;
D O I
10.1080/08860220701219863
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Creatol (CTL) is a hydroxyl radical adduct of creatinine (Cr). The serum methylguanidine (MG) level and the MG/Cr molar ratio are reported to be biomarkers for oxidative stress. The aim of this study was to examine whether urinary excretion of CTL, another oxidative stress-related marker, is increased in patients with chronic renal failure (CRF). One hundred twenty-four non-dialyzed patients with chronic renal failure (serum Cr level, 1.3-10.0 mg/dL) were recruited from our hospitals. Urine and serum levels of CTL and MG were determined by high-performance liquid chromatography with the use of 9, 10- phenanthrenequinone as a fluorogenic reagent. The CTL/Cr and (CTL+MG)/Cr molar ratios in spot urine samples were also compared with those in 24-h urine samples. The urinary CTL/Cr and (CTL+MG)/Cr molar ratios increased with decreases in Cr clearance in patients with CRF. Correlations between serum and spot urine (CTL+MG)/Cr and between serum and spot urine CTL/Cr were quite similar to those in 24-h urine samples. CTUCr and (CTL+MG)/Cr molar ratios in both 24-h urine and spot urine samples appear to be useful indices of the severity of CRF.
引用
收藏
页码:279 / 283
页数:5
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