Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers

被引：13

作者：

Carmon, G. ^{[1
,2
]}

Fishov, I. ^{[3
]}

Feingold, M. ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Ilse Katz Ctr Nanotechnol, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

来源：

OPTICS LETTERS | 2012年 / 37卷 / 03期

关键词：

ESCHERICHIA-COLI; Z RING; MICROSCOPY; DYNAMICS;

D O I：

10.1364/OL.37.000440

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments. (C) 2012 Optical Society of America

引用

页码：440 / 442

页数：3

共 16 条

[1] Bacterial cell division: assembly, maintenance and disassembly of the Z ring [J].