Methylophilus glucosoxydans sp nov., a restricted facultative methylotroph from rice rhizosphere

Two restricted facultatively methylotrophic strains, designed B-T and P, were isolated from rice roots. The isolates were strictly aerobic, Gram-negative, asporogenous, mesophilic, neutrophilic, motile rods that multiplied by binary fission and were able to synthesize indole-3-acetate. The cellular fatty acid profiles of the two strains were dominated by C-16 : 0, C-16 : 1 omega 7C and C-16 : 0 2-OH. The major ubiquinone was Q-8. The predominant phospholipids were phosphatidylethanolamine and phosphatidylglycerol. Cardiolipin (diphosphatidylglycerol) was absent. The two strains assimilated methanol carbon at the level of formaldehyde via the ribulose monophosphate cycle (2-keto-3-deoxy-6-phosphogluconate variant). They lacked alpha-ketoglutarate dehydrogenase and glutamate dehydrogenase. They assimilated ammonium via the glutamate cycle enzymes glutamine synthetase and glutamate synthase. The DNA G + C contents of strains B-T and P were 52.5 and 51.5 mol% (T-m,), respectively. The level of DNA DNA reassociation between these strains was 78%, indicating that they belong to one species. Phylogenetic analysis of strain B-T based on 16S rRNA and methanol dehydrogenase (mxaF) gene sequences showed a high level of similarity to members of the genus Methylophilus. As the two isolates were clearly distinct from all recognized members of the genus Methylophilus based on phenotypic data and levels of DNA DNA relatedness (30-46%), they are considered to represent a novel species, for which the name Methylophilus glucosoxydans sp. nov. is proposed; the type strain is B-T (=VKM B-1607(T)=CCUG 59685(T)=DSM 5898(T)).