Nanoplasmonics for Dual-Molecule Release through Nanopores in the Membrane of Red Blood Cells

被引：129

作者：

Delcea, Mihaela ^{[1
,2
]}

Sternberg, Nadine ^{[3
]}

Yashchenok, Alexey M. ^{[1
]}

Georgieva, Radostina ^{[3
,4
]}

Baeumler, Hans ^{[3
]}

Moehwald, Helmuth ^{[1
]}

Skirtach, Andre G. ^{[1
,5
,6
]}

机构：

[1] Max Planck Inst Colloids & Interfaces, Dept Interfaces, D-14424 Golm, Germany

[2] Ernst Moritz Arndt Univ Greifswald, ZIK HIKE, D-17489 Greifswald, Germany

[3] Charite Univ Med Berlin, Berlin Brandenburg Ctr Regenerat Therapies, Ctr Tumor Med, D-10117 Berlin, Germany

[4] Trakia Univ, Fac Med, Dept Med Phys Biophys & Radiol, Stara Zagora 6000, Bulgaria

[5] Univ Ghent, Dept Mol Biotechnol, B-9000 Ghent, Belgium

[6] Univ Ghent, NB Photon, B-9000 Ghent, Belgium

来源：

ACS NANO | 2012年 / 6卷 / 05期

关键词：

nanoplasmonics; trans-membrane; channel proteins; lipid bilayers; gold nanoparticles; localized heating; RBC; nanopores; GOLD NANOPARTICLES; OPTICAL-PROPERTIES; DRUG-DELIVERY; TARGETED TRANSFECTION; METAL NANOPARTICLES; COLORECTAL-CANCER; IN-VITRO; HEAT; LIGHT; MICROCAPSULES;

D O I：

10.1021/nn3006619

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A nanoplasmonics-based opto-nanoporation method of creating nanopores upon laser illumination is applied for inducing diffusion and triggered release of small and large molecules from red blood cells (RBCs). The method is Implemented using absorbing gold nanoparticle (Au-NP) aggregates on the membrane of loaded RBCs, which, upon near-IR laser light absorption, induce release of encapsulated molecules from selected cells. The binding of Au-NPs to RBCs is characterized by Raman spectroscopy. The process of release Is driven by heating localized at nanoparticles, which impacts the permeability of the membrane by affecting the lipid bilayer and/or trans-membrane proteins. Localized heating and temperature rise around Au-NP aggregates is simulated and discussed. Research reported in this work is relevant for generating nanopores for biomolecule trafficking through polymeric and lipid membranes as well as cell membranes, while dual- and multi-molecule release is relevant for theragnostics and a wide range of therapies.

引用

页码：4169 / 4180

页数：12

共 126 条

[1] Gold nanoparticle-incorporated human red blood cells (RBCs) for X-ray dynamic imaging [J].