Micellar electrokinetic chromatography of tri aza aromatic ligand compounds of iron (II): Influence of bile salt type on enantiomeric separation

被引：0

作者：

Elshihabi, S ^{[1
]}

Black, KD ^{[1
]}

Sutton, JK ^{[1
]}

Woody, KA ^{[1
]}

Burke, JA ^{[1
]}

Bushey, MM ^{[1
]}

机构：

[1] Trinity Univ, Dept Chem, San Antonio, TX 78212 USA

来源：

ELECTROPHORESIS | 2001年 / 22卷 / 17期

关键词：

micellar electrokinetic chromatography; bile salts; metal-ligand complexes; chiral separations;

D O I：

10.1002/1522-2683(200109)22:17<3771::AID-ELPS3771>3.0.CO;2-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Micellar electrokinetic chromatography is used with a variety of bile salt micelles to separate the enantiomers of bis(8-((pyridine-2-methylene)amino)quinoline)iron(Il) hexafluorophosphate, Fe(PMAQ)(2)(PF6)(2); bis(8-((pyridine-2-methylene)amino)lepidine iron(Il) hexafluorophosphate, Fe(PMAL)(2)(PF6)(2); and bis(1-(2-pyridinyl)ethylidine)-8-aminoquinoline iron(Il) hexafluorophosphate, Fe(PEAQ)(2)(PF6)(2). The influence of ten different bile salts on the resolution of each pair of enantiomers is investigated. Significant changes in resolution are seen depending upon the bile salt used. The dihydroxy bile salts are superior to the trihydroxy bile salts in terms of resolution, and the taurine or glycine conjugated bile salts yield better results than the unconjugated bile salts. Resolution for most enantiomers is maximized in a buffer solution containing 10-15% acetone and employing either taurochenodeoxycholic or glycochenodeoxycholic acid as the bile salt. Evidence for the separation of the corresponding Fe(III) complexes is presented.

引用

页码：3771 / 3777

页数：7

共 34 条

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