Structural elucidation of polysaccharide containing 3-O-methyl galactose from fruiting bodies of Pleurotus citrinopileatus

被引：24

作者：

He, Pengfei ^{[1
,2
]}

Zhang, Anqiang ^{[2
,3
,4
,5
]}

Zhou, Saijing ^{[2
]}

Zhang, Fuming ^{[3
,4
,5
]}

Linhardt, Robert J. ^{[3
,4
,5
,6
]}

Sun, Peilong ^{[2
]}

机构：

[1] Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Hangzhou 310014, Zhejiang, Peoples R China

[2] Zhejiang Univ Technol, Dept Food Sci & Technol, Hangzhou 310014, Zhejiang, Peoples R China

[3] Rensselaer Polytech Inst, Dept Chem, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[4] Rensselaer Polytech Inst, Dept Biol Chem, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[5] Rensselaer Polytech Inst, Dept Biomed Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[6] Rensselaer Polytech Inst, Dept Biol Sci, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

来源：

CARBOHYDRATE RESEARCH | 2016年 / 434卷

关键词：

Edible mushrooms; Pleurotus citrinopileatus; Polysaccharide; Structure; 3-O-methylated galactopyranose; ANTITUMOR POLYSACCHARIDES; EDIBLE MUSHROOM; IDENTIFICATION; MANNOGALACTAN; EXTRACTS; GLUCAN;

D O I：

10.1016/j.carres.2016.08.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A water-soluble polysaccharide containing 3-O-methyl galactose (PCP60W) was isolated from fruiting bodies of Pleurotus citrinopileatus and purified by anion-exchange and gel column chromatography. This polysaccharide has an average molecular weight of 2.74 x 10(4) Da and its structure was elucidated using monosaccharide composition and methylation analysis combined with one- and two-dimensional (COSY, TOCSY, NOESY, HMQC and HMBC) NMR spectroscopy. PCP60W was shown to be a linear partially 3-O-methylated alpha-galactopyranan comprised of 6-linked galactose, 6-linked 3-O-methyl galactose and 4 linked glucose in a ratio of 3.0:1.0:0.6. This work provides additional evidence for the view that 3-O-methyl galactose is common to the genus Pleurotus. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：72 / 76

页数：5

共 20 条

[1] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].